Nanofiber-acellular dermal matrix as a bilayer scaffold containing mesenchymal stem cell for healing of full-thickness skin wounds

Full-thickness skin defect is one of the main clinical problems, which cannot be repaired spontaneously. The aim of this study was to evaluate the feasibility of combining nanofibers with ADM as a bilayer scaffold for treatment of full-thickness skin wounds in a single-step procedure. The nanofibrou...

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Bibliographic Details
Published in:Cell and tissue research 2019-03, Vol.375 (3), p.709-721
Main Authors: Mirzaei-parsa, Mohamad Javad, Ghanbari, Hossein, Alipoor, Behnam, Tavakoli, Amirhossein, Najafabadi, Mohammad Reza H., Faridi-Majidi, Reza
Format: Article
Language:English
Subjects:
Adipose tissue
Analysis
Angiogenesis
Biomedical and Life Sciences
Biomedicine
Cell adhesion
Cell adhesion & migration
Cell proliferation
Collagen
EDTA
Electron microscopy
Fibrinogen
Flow cytometry
Human Genetics
Mesenchyme
Microscopy
Molecular Medicine
Polycaprolactone
Proteomics
Regular Article
Scanning electron microscopy
Skin
Skin & tissue grafts
Stem cell transplantation
Stem cells
Tissue engineering
Wound healing
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Summary:Full-thickness skin defect is one of the main clinical problems, which cannot be repaired spontaneously. The aim of this study was to evaluate the feasibility of combining nanofibers with ADM as a bilayer scaffold for treatment of full-thickness skin wounds in a single-step procedure. The nanofibrous polycaprolactone/fibrinogen scaffolds were fabricated by electrospinning. Subsequently, mesenchymal stem cells were isolated from rat adipose tissues and characterized by flow cytometry. Cell adhesion, proliferation, and the epidermal differentiation potential of adipose-derived stem cells (ADSCs) on nanofibrous scaffolds were investigated by scanning electron microscopy (SEM), alamarBlue, and real-time PCR, respectively. In animal studies, full-thickness excisional wounds were created on the back of rats and treated with following groups: ADM, ADM-ADSCs, nanofiber, nanofiber-ADSCs, bilayer, and bilayer-ADSCs. In all groups, wounds were harvested on days 14 and 21 after treatment to evaluate re-epithelialization, blood vessel density, and collagen content. The results indicated that ADSCs seeded on ADM, nanofiber, and bilayer scaffolds can promote re-epithelialization, angiogenesis, and collagen remodeling in comparison with cell-free scaffolds. In conclusion, nanofiber-ADSCs showed the best results for re-epithelialization (according to histological scoring), average blood vessel density (92.7 ± 6.8), and collagen density (87.4 ± 4.9%) when compared to the control and other experimental groups.
ISSN:0302-766X
1432-0878
DOI:10.1007/s00441-018-2927-6