Cover Picture: Site-Specific Protein Modification on Living Cells Catalyzed by Sortase (ChemBioChem 5/2008)

The cover picture shows a site-specific cell-surface protein modification catalyzed by Sortase A (SrtA), a transpeptidase from Staphylococcus aureus. As a model cell-surface protein, osteoclast differentiation factor (ODF), a type II membrane protein, was genetically modified at the C terminus with...

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Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2008-03, Vol.9 (5), p.653-653
Main Authors: Tanaka, Tsutomu, Yamamoto, Teruyasu, Tsukiji, Shinya, Nagamune, Teruyuki
Format: Article
Language:English
Subjects:
cell surface engineering
peptide tags
protein-protein conjugation
site-specific labeling
Sortase
Staphylococcus aureus
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Summary:The cover picture shows a site-specific cell-surface protein modification catalyzed by Sortase A (SrtA), a transpeptidase from Staphylococcus aureus. As a model cell-surface protein, osteoclast differentiation factor (ODF), a type II membrane protein, was genetically modified at the C terminus with a short peptide tag LPETGG, a recognition sequence of SrtA, and expressed on a living cell surface. Addition of SrtA and N-terminal triglycine-containing probes (biotin, Alexa Fluor 488, EGFP) to culture medium allowed site-specific labeling of the LPETGG-tagged protein on the living cell surface. The present strategy will provide important tools for cell biology and cell-surface engineering. For more information, see the article by T. Nagamune et al. on p. 802 ff.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.200890013