Isolation and Characterisation of a [Delta]5-fatty Acid Elongase from the Marine Microalga Pavlova salina

The marine microalga Pavlova salina (Haptophyta, Pavlovophyceae) produces lipids containing approximately 50% n-3 long-chain polyunsaturated fatty acids including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). A full-length cDNA sequence, designated PsElo5, was isolated from P. salina....

Full description

Saved in:
Bibliographic Details
Published in:Marine biotechnology (New York, N.Y.) N.Y.), 2009-06, Vol.11 (3), p.410-418
Main Authors: Robert, Stanley S, Petrie, James R, Zhou, Xue-rong, Mansour, Maged P, Blackburn, Susan I, Green, Allan G, Singh, Surinder P, Nichols, Peter D
Format: Article
Language:English
Subjects:
Algae
Biosynthesis
Enzymes
Fatty acids
Genes
Haptophyta
Lipids
Marine
Marine biology
Microalgae
Pavlova salina
Pavlovophyceae
Polyunsaturated fatty acids
Seafood
Seeds
Studies
Yeasts
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The marine microalga Pavlova salina (Haptophyta, Pavlovophyceae) produces lipids containing approximately 50% n-3 long-chain polyunsaturated fatty acids including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). A full-length cDNA sequence, designated PsElo5, was isolated from P. salina. Sequence alignment showed that the gene was homologous to corresponding ELO-type elongases from other microalgae. Heterologous expression of PsElo5 in yeast and in higher plants confirmed that it encodes a specific Δ5-elongase activity as predicted and, furthermore, within the n-3 pathway, the elongation activity was confined exclusively to EPA. [PUBLICATION ABSTRACT]
ISSN:1436-2228
1436-2236
DOI:10.1007/s10126-008-9157-y