Association between Her2/neu status in colorectal carcinoma and clinicopathological features: a retrospective study using whole - tissue sections

The human epidermal growth factor receptor 2 (Her2/neu) signal pathway plays a significant role in the occurance of various solid tumor types. The rate of Her2/neu in colorectal carcinoma (CRC) is not clearly elucidated. In this study, we discuss the association between Her2/neu overexpression and c...

Full description

Saved in:
Bibliographic Details
Published in:Polish journal of pathology 2018, Vol.69 (2), p.143-149
Main Authors: Kılıçarslan, Aydan, Dogan, Hayriye Tatli, Süngü, Nuran, Dogan, Mehmet, Yalcin, Abdussamet, Dede, Didem Şener
Format: Article
Language:English
Subjects:
Antigens
Breast cancer
Cancer therapies
Chemotherapy
Colon
Colorectal Neoplasms - genetics
Epidermal growth factor
Gastric cancer
Gene Amplification
Humans
Immunohistochemistry
Lymphatic system
Metastasis
Monoclonal antibodies
Receptor, ErbB-2 - genetics
Retrospective Studies
Tumors
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The human epidermal growth factor receptor 2 (Her2/neu) signal pathway plays a significant role in the occurance of various solid tumor types. The rate of Her2/neu in colorectal carcinoma (CRC) is not clearly elucidated. In this study, we discuss the association between Her2/neu overexpression and clinicopathological parameters in CRC. Her2/neu immunohistochemical (IHC) staining was performed on whole sections of formalin fixed paraffin embedded tumor tissues of 100 CRC resections. Cases with score 3+ and score 2+ expressions were further evaluated by silver in-situ hybridization (ISH) for the existence of Her2/neu gene amplification. Her2/neu membranous overexpression was observed in 12 of the 100 cases (6 cases with a score 3+ and 6 cases with a score 2+) and 6 of these were heterogenous. There were 10 cases with Her2/neu amplification (6/6 score 3+, 4/6 score 2+). Strong cytoplasmic staining was observed in 5 cases. Membranous scores were either 3+ or 2+ in 3 of these 5 cases. Moreover, there was Her2/neu amplification in 2 of these 3 cases. Her2/neu amplification status and overexpression was not related to clinicopathological parameters and overall survival. More clear results can be obtained from studies with Her2/neu IHC and ISH test on whole sections.
ISSN:1233-9687
2084-9869
DOI:10.5114/pjp.2018.75671