Bioresorbable electrospun gelatin/polycaprolactone nanofibrous membrane as a barrier to prevent cardiac postoperative adhesion

• Published in: Acta biomaterialia 2019-01, Vol.83, p.211-220
• Main Authors: Feng, Bei, Wang, Shoubao, Hu, Dongjian, Fu, Wei, Wu, Jinglei, Hong, Haifa, Domian, Ibrahim J., Li, Fen, Liu, Jinfen
• Format: Article
• Language: English
• Subjects: Adhesion; Animals; Barriers; Biocompatibility; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Biomedical materials; Bioresorbable; Cardiovascular disease; Congenital cardiac disease; Congenital diseases; Coronary artery disease; Disease Models, Animal; Electrospinning; Fibroblasts; Fibroblasts - metabolism; Fibroblasts - pathology; Gelatin; Gelatin - chemistry; Gelatin - pharmacology; Gelatin/polycaprolactone membrane; Heart diseases; Heart surgery; Infiltration; Mechanical properties; Membranes; Membranes, Artificial; Morphology; Myocardium - metabolism; Myocardium - pathology; Nanofibers - chemistry; Nanofibers - therapeutic use; Polycaprolactone; Polyesters - chemistry; Polyesters - pharmacology; Postoperative adhesions; Rabbits; Surgery; Surgical implants; Tissue Adhesions - metabolism; Tissue Adhesions - pathology; Tissue Adhesions - prevention & control
• ISSN: 1742-7061; 1878-7568
• DOI: 10.1016/j.actbio.2018.10.022

[Display omitted] Post-cardiac surgical sternal and epicardial adhesions increase the risk and complexity of cardiac re-operative surgeries, which represent a significant challenge for patients with the congenital cardiac disease. Bioresorbable membranes can serve as barriers to prevent postoperative adhesions. Herein, we fabricated a bioresorbable gelatin/polycaprolactone (GT/PCL) composite membrane via electrospinning. The membrane was characterized in terms of morphology, mechanical properties, and biocompatibility. We then evaluated its efficacy as a physical barrier to prevent cardiac operative adhesions in a rabbit model. Our results showed that the membrane had a nanofibrous structure and was sturdy enough to be handled for the surgical procedures. In vitro studies with rabbit cardiac fibroblasts demonstrated that the membrane was biocompatible and inhibited cell infiltration. Further application of the membrane in a rabbit cardiac adhesion model revealed that the membrane was resorbed gradually and effectively resisted the sternal and epicardial adhesions. Interestingly, six months after the operation, the GT/PCL membrane was completely resorbed with simultaneous ingrowth of host cells to form a natural barrier. Collectively, these results indicated that the GT/PCL membrane might be a suitable barrier to prevent sternal and epicardial adhesions and might be utilized as a novel pericardial substitute for cardiac surgery. Electrospinning is a versatile method to prepare nanofibrous membranes for tissue engineering and regenerative medicine applications. However, with the micro-/nano-scale structure and high porosity, the electrospun membrane might be an excellent candidate as a barrier to prevent postoperative adhesion. Here we prepared an electropun GT/PCL nanofibrous membrane and applied it as a barrier to prevent sternal and epicardial adhesions. Our results showed that the membrane had sufficient mechanical strength, good biocompatibility, and effectively resisted the sternal and epicardial adhesions. What’s more, the membrane was bioresorbable and allowed simultaneous ingrowth of host cells to form a natural barrier. We believe that the current will inspire more research on nanomaterials to prevent postoperative adhesion applications.