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A simple and highly efficient method for transduction of human adipose‐derived mesenchymal stem cells

Mesenchymal stem cells (MSCs) are multipotent cells capable of differentiating into a wide range of cell types and provide a potential to transfer therapeutic protein in vivo, making them valuable candidates for gene therapy and cell therapy. However, using MSCs in in vivo is limited due to the low...

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Bibliographic Details
Published in:Journal of cellular biochemistry 2019-02, Vol.120 (2), p.1726-1734
Main Authors: Bolandi, Zohreh, Hosseini Rad, Seyed Mohammad Ali, Soudi, Sara, Hashemi, Seyed Mahmoud, Ghanbarian, Hossein
Format: Article
Language:English
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Summary:Mesenchymal stem cells (MSCs) are multipotent cells capable of differentiating into a wide range of cell types and provide a potential to transfer therapeutic protein in vivo, making them valuable candidates for gene therapy and cell therapy. However, using MSCs in in vivo is limited due to the low rate of transfection and transduction efficacy. Therefore, developing methods to efficiently transfer genes into MSCs would provide a number of opportunities for using them in the clinic. Here, we introduce a simple and robust method for efficient transduction of human adipose‐derived MSCs by modification under the culture condition of human embryonic kidney cells 293 (HEK293T) and MSCs. Moreover, as a transduction enhancer, polybrene was replaced with Lipofectamine, a cationic lipid. Therefore, we showed that transduction of primary cells can be increased efficiently by modifying the culture condition. Mesenchymal stem cells (MSCs) are valuable candidates for gene‐ and cell‐based therapies. However, using MSCs in in vivo is limited due to the low rate of transfection and transduction efficacy. Here, we developed a novel and efficient method by modifying transduction conditions. Significantly, our optimized virus production and transduction procedure showed that MSCs could be transduced simply, directly, and more efficiently without using polybrene and ultracentrifugation.
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.27453