Demonstration of catalytic proton acceptor of chitosanase from Paenibacillus fukuinensis by comprehensive analysis of mutant library

Chitosanase from Paenibacillus fukuinensis D2 is an attractive enzyme, and it exhibits both chitosanase and β-1, 4 glucanase activities. In our previous study, we generated P. fukuinensis chitosanase-displaying yeast cells using a yeast cell surface-displaying system. Chitosanase-displaying yeast ca...

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Published in:Applied microbiology and biotechnology 2009-11, Vol.85 (1), p.95-104
Main Authors: Isogawa, Danya, Fukuda, Takeshi, Kuroda, Kouichi, Kusaoke, Hideo, Kimoto, Hisashi, Suye, Shin-ichiro, Ueda, Mitsuyoshi
Format: Article
Language:English
Subjects:
Amino Acid Substitution - genetics
Amino acids
Asparagine - genetics
Asparagine - metabolism
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biological and medical sciences
Biotechnologically Relevant Enzymes and Proteins
Biotechnology
Cellulase
Cellulose
Chitosan - metabolism
Crustaceans
DNA Mutational Analysis
E coli
Enzymes
Fundamental and applied biological sciences. Psychology
Glucans - metabolism
Glutamine - genetics
Glutamine - metabolism
Glycoside Hydrolases - genetics
Glycoside Hydrolases - metabolism
Libraries
Life Sciences
Microbial Genetics and Genomics
Microbiology
Models, Molecular
Mutagenesis, Site-Directed
Paenibacillus - enzymology
Paenibacillus - genetics
Paenibacillus fukuinensis
Plasmids
Protein Structure, Tertiary
Protons
Studies
Yeast
Yeasts
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cited_by cdi_FETCH-LOGICAL-c521t-3f8f56a4a18a9d7ee7743a5d1c3b5fb8a05e43c57a4a51829ff0ccbe50f9efd33
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container_title Applied microbiology and biotechnology
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creator Isogawa, Danya
Fukuda, Takeshi
Kuroda, Kouichi
Kusaoke, Hideo
Kimoto, Hisashi
Suye, Shin-ichiro
Ueda, Mitsuyoshi
description Chitosanase from Paenibacillus fukuinensis D2 is an attractive enzyme, and it exhibits both chitosanase and β-1, 4 glucanase activities. In our previous study, we generated P. fukuinensis chitosanase-displaying yeast cells using a yeast cell surface-displaying system. Chitosanase-displaying yeast can be utilized as a chitosanase cluster without many time-consuming purification steps. In this study, using the system, we have investigated whether Glu302, which is supposed as a putative proton acceptor, is an essential amino acid residue for exhibiting chitosanase activity and analyzed the contribution of mutual interaction between Glu302 and Asn312 to the activity. A mutant library in which Glu302 and Asn312 were comprehensively substituted by the other amino acid residues was constructed on the yeast cell surface. From the results of chitosanase and β-1, 4 glucanase activity assays, we demonstrated that Glu302 was a proton acceptor for chitosanase activity, and Asn312 also participated in the hydrolysis of chitosan and cellulose.
doi_str_mv 10.1007/s00253-009-2041-5
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In our previous study, we generated P. fukuinensis chitosanase-displaying yeast cells using a yeast cell surface-displaying system. Chitosanase-displaying yeast can be utilized as a chitosanase cluster without many time-consuming purification steps. In this study, using the system, we have investigated whether Glu302, which is supposed as a putative proton acceptor, is an essential amino acid residue for exhibiting chitosanase activity and analyzed the contribution of mutual interaction between Glu302 and Asn312 to the activity. A mutant library in which Glu302 and Asn312 were comprehensively substituted by the other amino acid residues was constructed on the yeast cell surface. From the results of chitosanase and β-1, 4 glucanase activity assays, we demonstrated that Glu302 was a proton acceptor for chitosanase activity, and Asn312 also participated in the hydrolysis of chitosan and cellulose.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>19517107</pmid><doi>10.1007/s00253-009-2041-5</doi><tpages>10</tpages></addata></record>
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subjects Amino Acid Substitution - genetics
Amino acids
Asparagine - genetics
Asparagine - metabolism
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biological and medical sciences
Biotechnologically Relevant Enzymes and Proteins
Biotechnology
Cellulase
Cellulose
Chitosan - metabolism
Crustaceans
DNA Mutational Analysis
E coli
Enzymes
Fundamental and applied biological sciences. Psychology
Glucans - metabolism
Glutamine - genetics
Glutamine - metabolism
Glycoside Hydrolases - genetics
Glycoside Hydrolases - metabolism
Libraries
Life Sciences
Microbial Genetics and Genomics
Microbiology
Models, Molecular
Mutagenesis, Site-Directed
Paenibacillus - enzymology
Paenibacillus - genetics
Paenibacillus fukuinensis
Plasmids
Protein Structure, Tertiary
Protons
Studies
Yeast
Yeasts
title Demonstration of catalytic proton acceptor of chitosanase from Paenibacillus fukuinensis by comprehensive analysis of mutant library
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