Identifying macrophage enrichment in atherosclerotic plaques by targeting dual-modal US imaging/MRI based on biodegradable Fe-doped hollow silica nanospheres conjugated with anti-CD68 antibody

Macrophage recruitment has emerged as the crucial force driving the initiation and progression of atherosclerotic lesions. Therefore, the identification of macrophages in plaques is of vital importance for identifying vulnerable plaques, and noninvasive imaging methods are particularly desirable. So...

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Bibliographic Details
Published in:Nanoscale 2018-11, Vol.10 (43), p.20246-20255
Main Authors: Ji, Ri, Li, Xiaoyu, Zhou, Chun, Tian, Qiwei, Li, Chang, Xia, Shujun, Wang, Ronghui, Feng, Yun, Zhan, Weiwei
Format: Article
Language:English
Subjects:
Animals
Antibodies - chemistry
Antibodies - immunology
Antigens, CD - immunology
Antigens, Differentiation, Myelomonocytic - immunology
Aorta
Apolipoproteins E - deficiency
Apolipoproteins E - genetics
Atherosclerosis
Atherosclerosis - diagnosis
Atherosclerosis - diagnostic imaging
Atherosclerosis - immunology
Biocompatibility
Biodegradability
Cell Line
Cell Survival - drug effects
Contrast agents
Contrast Media - chemistry
Ferrous Compounds - chemistry
Humans
Identification methods
Immunofluorescence
Iron
Lesions
Macrophages
Macrophages - cytology
Macrophages - metabolism
Macrophages - pathology
Magnetic resonance imaging
Magnetic Resonance Imaging - methods
Mice
Mice, Knockout
Microscopy, Confocal
Nanoparticles
Nanospheres
Nanostructures - chemistry
Nanostructures - toxicity
Silicon dioxide
Silicon Dioxide - chemistry
Ultrasonography
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Summary:Macrophage recruitment has emerged as the crucial force driving the initiation and progression of atherosclerotic lesions. Therefore, the identification of macrophages in plaques is of vital importance for identifying vulnerable plaques, and noninvasive imaging methods are particularly desirable. Some studies have reported that MRI can detect plaque macrophages through targeted nanoparticles, but it is still hard for an US to detect macrophages in atherosclerotic plaque. In this study, anti-CD68 receptor-targeted Fe-doped hollow silica nanoparticles (CD68-Fe-HSNs) were fabricated as a dual-modal US/MRI contrast agent for identifying macrophages of aorta ventralis atherosclerotic plaques in ApoE-/- mice, confirmed by immunofluorescence and bio-TEM. This system possesses biodegradable characteristics even though it is an inorganic mesoporous nanosystem, indicating its potential high biocompatibility for further in vivo research. We expect that these dual-modal US/MRI nanoparticles will play a role in assessing vulnerable plaque in future research studies.
ISSN:2040-3364
2040-3372
DOI:10.1039/c8nr04703k