Diversity and metallo-β-lactamase-producing genes in Pseudomonas aeruginosa strains isolated from filters of household water treatment systems

• Published in: Journal of environmental management 2019-02, Vol.231, p.413-418
• Main Authors: Mombini, Sara, Rezatofighi, Seyedeh Elham, Kiyani, Laleh, Motamedi, Hossein
• Format: Article
• Language: English
• Subjects: Household water treatment system; Metallo- -lactamase; Pseudomonas aeruginosa; RAPD-PCR
• ISSN: 0301-4797; 1095-8630
• DOI: 10.1016/j.jenvman.2018.10.068

The microbiological quality of drinking water has long been a critical element in public health. Considering the high clinical relevance of Pseudomonas aeruginosa, we examined the filters of household water treatment systems for its presence and characteristics to determine the systems' efficiency in eliminating the bacteria. In total, filters of 50 household water treatment systems were examined. Microbiological and molecular methods were used for the detection and confirmation of P. aeruginosa isolates. Random Amplification of Polymorphic DNA-polymerase chain reaction (RAPD-PCR) was performed to detect similarities and differences among P. aeruginosa isolates. Combined disk (CD) method and double disk synergy test (DDST) were performed to detect metallo-beta-lactamase (MBL)-producing P. aeruginosa isolates. Finally, PCR was performed to detect MBL genes in MBL-producing strains. From the 50 analyzed systems, 76 colonies of P. aeruginosa were identified. In some systems, isolated bacteria from different filters harbored similar genetic profiles, indicating that these isolates may be able to pass through the filter and reach higher filters of the system. Phenotypic tests revealed 7 (9.2%) MBL-producing strains. Two isolates were positive for blaVIM-1, whereas one isolate was positive for blaNDM and blaIMP-1. The wide distribution of resistant phenotypes and genetic plasticity of these bacteria in household water treatment systems indicate that resistance mechanisms circulate among P. aeruginosa isolates in the environment of the filtration systems. The presence of MBL-producing genes in these systems and P. aeruginosa as a potential reservoir of these resistance genes can be a major concern for public health. •Pseudomonas aeruginosa has high density and diversity in filters of household water treatment systems.•Resistant phenotypes and MBL-producing genes are distributed in these systems.•More than 39% of P. aeruginosa isolates were resistant to ticarcillin.•Household water treatment systems cannot completely eliminate these bacteria.