Day‐7 embryos generate an anti‐inflammatory immune response in peripheral blood immune cells in superovulated cows

• Published in: American journal of reproductive immunology (1989) 2019-02, Vol.81 (2), p.e13069-n/a
• Main Authors: Talukder, Anup K., Rashid, Mohammad B., Takedomi, Toshiro, Moriyasu, Satoru, Imakawa, Kazuhiko, Miyamoto, Akio
• Format: Article
• Language: English
• Subjects: 2',5'-Oligoadenylate Synthetase - genetics; Animals; Cattle; Day‐7 embryo; Embryo transfer; Embryo, Mammalian; Embryos; Estrus; Female; Gene expression; Immune response; Immunity, Cellular - genetics; Inflammation; Inflammation - genetics; Inflammation - immunology; Insemination, Artificial; Interferon; Interleukin 1; Interleukin 10; Interleukin-10 - genetics; Leukocytes (mononuclear); Leukocytes (neutrophilic); Leukocytes (polymorphonuclear); Leukocytes, Mononuclear - physiology; Neutrophils - physiology; peripheral blood immune cells; Peripheral blood mononuclear cells; Pregnancy; Pregnancy - immunology; Prostaglandin-E Synthases - genetics; superovulated cows; Superovulation; Transforming Growth Factor beta1 - genetics; Transforming growth factor-b1; Transplantation Tolerance; Up-Regulation; Uterus
• ISSN: 1046-7408; 1600-0897
• DOI: 10.1111/aji.13069

Problem Recently, we demonstrated both in vitro and in vivo that an immunological crosstalk between Day‐7 embryo and immune cells exists locally in the uterus in cows. The peripheral immune response to early embryos at Day‐7 of pregnancy in cows remains largely unknown. Thus, we aimed to investigate the response of peripheral blood immune cells in the presence of multiple Day‐7 embryos in the uterus in donor cows of embryo transfer program. Method of study Superovulated cows were either inseminated (n = 13) at 12‐18 hours post‐estrus or remained without inseminations (n = 6). Blood was collected following insemination (Day‐1) and immediately after uterine flushing (Day‐7). Polymorphonuclear neutrophils (PMNs) and peripheral blood mononuclear cells (PBMCs) were isolated from whole blood for analysis of gene expression. Results Interferon‐stimulated genes (ISGs: ISG15 and OAS1) were increased in both PMNs and PBMCs, with up‐regulation of PTGES and anti‐inflammatory cytokines (TGFB1 and IL10) expressions at Day‐7 of post‐inseminations in cows, when compared to those at Day‐7 in non‐inseminated cows. Conclusion The findings indicate that the presence of multiple embryos in the uterus generates an anti‐inflammatory immune response in peripheral blood immune cells at Day‐7 of pregnancy in cows, which might help to accept semi‐allogenic embryo in the uterus.