Perfluorocarbon-Based 19 F MRI Nanoprobes for In Vivo Multicolor Imaging

• Published in: Angewandte Chemie International Edition 2018-12, Vol.57 (51), p.16742-16747
• Main Authors: Akazawa, Kazuki, Sugihara, Fuminori, Nakamura, Tatsuya, Matsushita, Hisashi, Mukai, Hiroaki, Akimoto, Rena, Minoshima, Masafumi, Mizukami, Shin, Kikuchi, Kazuya
• Format: Article
• Language: English
• Subjects: Contrast Media - chemistry; Fluorescent Dyes - chemistry; Fluorine - chemistry; Fluorocarbons - chemistry; Magnetic Resonance Imaging; Molecular Structure; Nanoparticles - chemistry; Optical Imaging; Silicon Dioxide - chemistry
• ISSN: 1433-7851; 1521-3773; 1521-3773
• DOI: 10.1002/anie.201810363

In vivo multicolor imaging is important for monitoring multiple biomolecular or cellular processes in biology. F magnetic resonance imaging (MRI) is an emerging in vivo imaging technique because it can non-invasively visualize F nuclei without endogenous background signals. Therefore, F MRI probes capable of multicolor imaging are in high demand. Herein, we report five types of perfluorocarbon-encapsulated silica nanoparticles that show F NMR peaks with different chemical shifts. Three of the nanoprobes, which show spectrally distinct F NMR peaks with sufficient sensitivity, were selected for in vivo multicolor F MRI. The nanoprobes exhibited F MRI signals with three colors in a living mouse. Our in vivo multicolor system could be utilized for evaluating the effect of surface functional groups on the hepatic uptake in a mouse. This novel multicolor imaging technology will be a practical tool for elucidating in vivo biomolecular networks by F MRI.