Mucilaginibacter xinganensis sp. nov., a phenanthrene-degrading bacterium isolated from wetland soil

• Published in: Antonie van Leeuwenhoek 2019-04, Vol.112 (4), p.641-649
• Main Authors: Jiang, Min-zhi, Qiu, Zhi-jun, Zhang, Guang-wu, Gao, Shi-yin, You, Xiao-yan, Ma, Ying-fei
• Format: Article
• Language: English
• Subjects: Bacterial Typing Techniques; Bacteroidetes - classification; Bacteroidetes - genetics; Bacteroidetes - isolation & purification; Bacteroidetes - metabolism; Biodegradation, Environmental; Biomedical and Life Sciences; Catalase; China; DNA, Bacterial - genetics; Fatty acids; Fatty Acids - chemistry; Fatty Acids - metabolism; Gene sequencing; Genomes; Life Sciences; Lipids; Medical Microbiology; Menaquinones; Microbiology; New species; Original Paper; Phenanthrene; Phenanthrenes - metabolism; Phosphatidylethanolamine; Phylogenetics; Phylogeny; Plant Sciences; Quinine; RNA, Ribosomal, 16S - genetics; rRNA 16S; Soil - chemistry; Soil bacteria; Soil Microbiology; Soil Science & Conservation; Wetlands
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-018-1194-6

An aerobic, Gram-stain negative, rod-shaped and non-motile strain, BJC16-A31 T , was isolated from the wetland soil sample taken from Daxing’anling, Heilongjiang, People’s Republic of China. Strain BJC16-A31 T was found to be oxidase- and catalase-positive, and produced light orange colonies on modified R2A agar. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BJC16-A31 T is closely related to Mucilaginibacter gotjawali SA3-7 T with 96.54% sequence similarity and it formed a separate lineage in the genus Mucilaginibacter . Strain BJC16-A31 T contained menaquinone-7 (MK-7) as the predominant isoprenoid quinine. Anteiso-C 15:0 , C 16:0 and anteiso-C 15:0 were the major fatty acids. The major polar lipids were phosphatidylethanolamine, six unidentified polar lipid, two unidentified aminophospholipids and one unidentified aminolipid. The genome is composed of a circular 5,301,339 bp chromosome with average G + C percentage of 42.25%. The Average Nucleotide Identity (ANI) between strain BJC16-A31 T and M. gotjawali SA3-7 T was 77.51%. Combined phenotypic, chemotaxonomic, phylogenetic and genomic characteristics support the conclusion that strain BJC16-A31 T represents a novel species of the genus Mucilaginibacter , for which the name Mucilaginibacter xinganensis sp. nov. is proposed. The type strain is BJC16-A31 T (= CGMCC 1.12728 T  = NBRC 110384 T ).