Unreliability of Results of PCR Detection of Helicobacter pylori in Clinical or Environmental Samples

The aim of this study was to compare published Helicobacter pylori primer pairs for their ability to reliably detect H. pylori in gastric biopsy specimens and salivary samples. Detection limits of the 26 PCR primer pairs previously described for detection of H. pylori DNA in clinical samples were de...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2009-03, Vol.47 (3), p.738-742
Main Authors: Sugimoto, Mitsushige, Wu, Jeng-Yih, Abudayyeh, Suhaib, Hoffman, Jill, Brahem, Hajer, Al-Khatib, Khaldun, Yamaoka, Yoshio, Graham, David Y
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
DNA Primers - genetics
Environmental Microbiology
False Positive Reactions
Fundamental and applied biological sciences. Psychology
Gastric Mucosa - microbiology
Helicobacter Infections - diagnosis
Helicobacter Infections - microbiology
Helicobacter pylori
Helicobacter pylori - genetics
Helicobacter pylori - isolation & purification
Humans
Microbiology
Miscellaneous
Polymerase Chain Reaction - methods
Saliva - microbiology
Sensitivity and Specificity
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Summary:The aim of this study was to compare published Helicobacter pylori primer pairs for their ability to reliably detect H. pylori in gastric biopsy specimens and salivary samples. Detection limits of the 26 PCR primer pairs previously described for detection of H. pylori DNA in clinical samples were determined. Sensitivity and specificity were determined using primers with detection limits of
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.01563-08