Molecular polymorphism between populations of Pseudoperonospora cubensis from Greece and the Czech Republic and their phytopathological and phylogenetic implications

Molecular genetic polymorphisms within Pseudoperonospora cubensis (Berk. and Curt.) Rostov. (Chromista, Peronosporales) isolates of different geographic origins were investigated to establish phylogenetic relationships. Thirty isolates originating from Greece (Crete; 15), Czech Republic (13), the Ne...

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Bibliographic Details
Published in:Phytopathologia mediterranea 2009-08, Vol.48 (2), p.316-316
Main Authors: Sarris, P F, Abdelhalim, M, Kitner, M V, Skandalis, N, Panopoulos, N J, Doulis, A G, Lebeda, A
Format: Article
Language:English
Subjects:
Chromista
Cucumis sativus
Peronosporales
Pseudoperonospora cubensis
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Summary:Molecular genetic polymorphisms within Pseudoperonospora cubensis (Berk. and Curt.) Rostov. (Chromista, Peronosporales) isolates of different geographic origins were investigated to establish phylogenetic relationships. Thirty isolates originating from Greece (Crete; 15), Czech Republic (13), the Netherlands and France (one isolate from each country) were analysed with two distinct molecular techniques (AFLP and ITS rDNA sequence analysis). All isolates were obtained from cucumber (Cucumis sativus L.) plants showing typical downy mildew symptoms. Sequence analysis of the ITS region of rDNA is a well established taxonomic tool within the genus Pseudoperonospora. However, AFLP typing has not been used previously for molecular polymorphism analysis in this species. AFLP fingerprinting produced ample polymorphisms and grouped isolates along two separate clusters; one included the Czech (Central Europe) and West European (the Netherlands, France) isolates, while the other only the isolates of Greek origin. Within each group there was some variation which could be accounted for by geographic origin and pathogenicity. ITS rDNA analysis showed no variability among isolates in ITS-1; however, all ITS-2 rDNA sequences of Greek and Czech isolates clustered together with isolates from Austria (NCBI access) forming a large cluster together with P. humuli indicating their close taxonomic relationship. Application of classical phytopathological and molecular approaches in the research of genetic structure and dynamics of P. cubensis populations is discussed.
ISSN:0031-9465