Sensing and reference pads for integrated optical immunosensors

For integrated optical immunosensors, a much more reliable interpretation of the sensor output signal can be achieved by introducing reference pads near the sensing pads, in order to separate specific from nonspecific effects. The results of theoretical and experimental investigations are reported f...

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Bibliographic Details
Published in:Journal of Biomedical Optics 1997-10, Vol.2 (4), p.391-400
Main Authors: Duebendorfer, Juerg, Kunz, Rino E, Schuermann, E, Duveneck, Gert L, Ehrat, Markus
Format: Article
Language:English
Subjects:
Bioassay
Chemical bonds
Immunosensors
Integrated optoelectronics
Molecules
Proteins
Refractive index
Signal processing
Temperature
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Summary:For integrated optical immunosensors, a much more reliable interpretation of the sensor output signal can be achieved by introducing reference pads near the sensing pads, in order to separate specific from nonspecific effects. The results of theoretical and experimental investigations are reported for immunosensors based on measuring changes of the effective refractive index due to the binding of analyte molecules. The emphasis is on the correction for variations of the light wavelength, the angle of incidence, and the temperature for integrated optical grating coupler chips. Using the binding of rabbit immunoglobulin to immobilized protein A as a regenerable model bioaffinity system, the influence of consecutive assay cycles on the two pads has been investigated. Experiments have been performed using sensor chips consisting of TiO sub(2) waveguiding films on fused silica and on replicated polycarbonate substrates.
ISSN:1083-3668
1560-2281
DOI:10.1117/12.281487