Development of a third-generation glucose sensor based on the open circuit potential for continuous glucose monitoring

• Published in: Biosensors & bioelectronics 2019-01, Vol.124-125, p.216-223
• Main Authors: Lee, Inyoung, Loew, Noya, Tsugawa, Wakako, Ikebukuro, Kazunori, Sode, Koji
• Format: Article
• Language: English
• Subjects: Biosensing Techniques; Blood Glucose - chemistry; Blood Glucose Self-Monitoring - methods; Continuous glucose monitoring (CGM); Diabetes Mellitus - blood; Diabetes Mellitus - pathology; Direct electron transfer; FAD dependent glucose dehydrogenase (FADGDH); Flavin-Adenine Dinucleotide - chemistry; Glucose - chemistry; Glucose - isolation & purification; Glucose 1-Dehydrogenase - chemistry; Humans; Insulin - chemistry; Insulin - metabolism; Open circuit potential; Third generation glucose sensor
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2018.09.099

Continuous glucose monitoring (CGM) systems are most important in the current Type I diabetes care and as component for the development of artificial pancreas systems because the amount of insulin being supplied is calculated based on the CGM results. Therefore, to stably and accurately control the blood glucose level, CGM should be stable and accurate for a long period. We have been engaged in the biomolecular engineering and application of FAD dependent glucose dehydrogenase complex (FADGDH) which is capable of direct electron transfer. In this study, we report the development of the third-generation type open circuit potential (OCP) principle-based glucose sensor with direct electron transfer FADGDH immobilized on gold electrodes using a self-assembled monolayer (SAM). We developed a novel algorithm for OCP-based glucose sensors. By employing this new algorithm, high reproducibility of measurement and sensor preparation were achieved. In addition, the signal was not affected by the presence of acetaminophen and ascorbic acid in the sample solution. The thus optimized third-generation OCP-based glucose sensor could be operated continuously for more than 9 days without significant change in the signal, sensitivity and dynamic range, indicating its potential application for CGM systems. •The third-generation type open circuit potential principle-based glucose sensor was developed.•A novel OCP measurement protocol, which discharge charged electron before measurement, was proposed.•This sensor showed high reproducibility in the measurement and electrode preparation.•This sensor signal was not affected by the presence of 340 µM ascorbic acid or 2.6 mM of acetaminophen in the sample solution.•This sensor could measure the glucose concentration stably and continuously during 9 days.