Expression of Cholera Toxin B Subunit in Transgenic Rice Endosperm

The synthetic cholera toxin B subunit (CTB) gene, modified according to the optimized codon usage of plant genes, was introduced into a plant expression vector and expressed under the control of the Bx17 HMW (high molecular weight) wheat endosperm-specific promoter containing an intron of the rice a...

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Bibliographic Details
Published in:Molecular biotechnology 2008-11, Vol.40 (3), p.261-268
Main Authors: Oszvald, Maria, Kang, Tae-Jin, Tomoskozi, Sandor, Jenes, Barnabas, Kim, Tae-Geum, Cha, Youn-Soo, Tamas, Laszlo, Yang, Moon-Sik
Format: Article
Language:English
Subjects:
Agricultural biotechnology
Biochemistry
Biological and medical sciences
Biological Techniques
Biotechnology
Blotting, Northern
Blotting, Western
Cell Biology
Chemistry
Chemistry and Materials Science
Cholera
Cholera Toxin - genetics
DNA, Plant - metabolism
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation, Plant
Genetic Vectors - genetics
Human Genetics
Oryza - genetics
Oryza sativa
Plants, Genetically Modified
Protein Science
Receptors, Cell Surface - metabolism
Rice
Seeds - genetics
Studies
Toxins
Transgenic plants
Triticum aestivum
Waterborne diseases
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Summary:The synthetic cholera toxin B subunit (CTB) gene, modified according to the optimized codon usage of plant genes, was introduced into a plant expression vector and expressed under the control of the Bx17 HMW (high molecular weight) wheat endosperm-specific promoter containing an intron of the rice act1 . The recombinant vector was transformed into rice plants using a biolistic-mediated transformation method. Stable integration of the synthetic CTB gene into the chromosomal DNA was confirmed by PCR amplification analysis. A high level of CTB (2.1% of total soluble protein) was expressed in the endosperm tissue of the transgenic rice plants. The synthetic CTB produced only in the rice endosperm demonstrated strong affinity for G M1 -ganglioside, thereby suggesting that the CTB subunits formed an active pentamer. The successful expression of CTB genes in transgenic plants makes it a powerful tool for the development of a plant-derived edible vaccine.
ISSN:1073-6085
1559-0305
DOI:10.1007/s12033-008-9083-2