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Enzyme-based bioartificial polymeric materials: the α-amylase-poly(vinyl alcohol) system
The possibility of realizing bioartificial polymeric materials from blends of the enzyme α‐amylase (EC 3.2.1.1) and poly(vinyl alcohol), in the form of film and hydrogel, was investigated. The interactions at molecular level between the enzyme and the synthetic polymer have been studied by calorimet...
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| Published in: | Polymer international 1997-12, Vol.44 (4), p.510-516 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| container_end_page | 516 |
| container_issue | 4 |
| container_start_page | 510 |
| container_title | Polymer international |
| container_volume | 44 |
| creator | Cristallini, Caterina Lazzeri, Luigi Cascone, Maria Grazia Polacco, Giovanni Lupinacci, Domenico Barbani, Niccoletta |
| description | The possibility of realizing bioartificial polymeric materials from blends of the enzyme α‐amylase (EC 3.2.1.1) and poly(vinyl alcohol), in the form of film and hydrogel, was investigated. The interactions at molecular level between the enzyme and the synthetic polymer have been studied by calorimetry, X‐ray diffractometry and morphological analysis. A certain degree of interaction through which the α‐amylase molecules influence the crystallinity of the poly(vinyl alcohol) was observed. The thermal stability of the enzyme in the blends was enhanced, while its biological activity did not vary significantly. In addition, hydrogels prepared by a freeze–thawing method can be considered suitable for manufacturing delivery systems capable of releasing enzymes which maintain their full biological activity. ©1997 SCI |
| doi_str_mv | 10.1002/(SICI)1097-0126(199712)44:4<510::AID-PI884>3.0.CO;2-0 |
| format | article |
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The interactions at molecular level between the enzyme and the synthetic polymer have been studied by calorimetry, X‐ray diffractometry and morphological analysis. A certain degree of interaction through which the α‐amylase molecules influence the crystallinity of the poly(vinyl alcohol) was observed. The thermal stability of the enzyme in the blends was enhanced, while its biological activity did not vary significantly. In addition, hydrogels prepared by a freeze–thawing method can be considered suitable for manufacturing delivery systems capable of releasing enzymes which maintain their full biological activity. ©1997 SCI</description><identifier>ISSN: 0959-8103</identifier><identifier>EISSN: 1097-0126</identifier><identifier>DOI: 10.1002/(SICI)1097-0126(199712)44:4<510::AID-PI884>3.0.CO;2-0</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Applied sciences ; bioartificial polymeric materials ; Biological and medical sciences ; Biotechnology ; Calorimetry ; delivery systems ; Enzymes ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Hydrogels ; Immobilization of enzymes and other molecules ; Immobilization techniques ; Methods. Procedures. Technologies ; Molecular dynamics ; Morphology ; Organic polymers ; Physicochemistry of polymers ; poly(vinyl alcohol) ; Polymer blends ; Polyvinyl alcohols ; Properties and characterization ; Special properties (catalyst, reagent or carrier) ; Thawing ; Thermodynamic stability ; X ray diffraction analysis ; α-amylase</subject><ispartof>Polymer international, 1997-12, Vol.44 (4), p.510-516</ispartof><rights>Copyright © 1997 SCI</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2121607$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Cristallini, Caterina</creatorcontrib><creatorcontrib>Lazzeri, Luigi</creatorcontrib><creatorcontrib>Cascone, Maria Grazia</creatorcontrib><creatorcontrib>Polacco, Giovanni</creatorcontrib><creatorcontrib>Lupinacci, Domenico</creatorcontrib><creatorcontrib>Barbani, Niccoletta</creatorcontrib><title>Enzyme-based bioartificial polymeric materials: the α-amylase-poly(vinyl alcohol) system</title><title>Polymer international</title><addtitle>Polym. Int</addtitle><description>The possibility of realizing bioartificial polymeric materials from blends of the enzyme α‐amylase (EC 3.2.1.1) and poly(vinyl alcohol), in the form of film and hydrogel, was investigated. The interactions at molecular level between the enzyme and the synthetic polymer have been studied by calorimetry, X‐ray diffractometry and morphological analysis. A certain degree of interaction through which the α‐amylase molecules influence the crystallinity of the poly(vinyl alcohol) was observed. The thermal stability of the enzyme in the blends was enhanced, while its biological activity did not vary significantly. In addition, hydrogels prepared by a freeze–thawing method can be considered suitable for manufacturing delivery systems capable of releasing enzymes which maintain their full biological activity. ©1997 SCI</description><subject>Applied sciences</subject><subject>bioartificial polymeric materials</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Calorimetry</subject><subject>delivery systems</subject><subject>Enzymes</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogels</subject><subject>Immobilization of enzymes and other molecules</subject><subject>Immobilization techniques</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular dynamics</subject><subject>Morphology</subject><subject>Organic polymers</subject><subject>Physicochemistry of polymers</subject><subject>poly(vinyl alcohol)</subject><subject>Polymer blends</subject><subject>Polyvinyl alcohols</subject><subject>Properties and characterization</subject><subject>Special properties (catalyst, reagent or carrier)</subject><subject>Thawing</subject><subject>Thermodynamic stability</subject><subject>X ray diffraction analysis</subject><subject>α-amylase</subject><issn>0959-8103</issn><issn>1097-0126</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqFkNtu1DAQhiMEEkvhHXKB0O6Ft-PEjuPlIJW0lEilWwkoh5uR43VUg7NZ4hQIb8WL8Ew4pNobkLgayf7n-0dfFD2lsKQAyeH8dVmUCwpSEKBJNqdSCposGFuxJ5zCanVUHpOLMs_Zs3QJy2L9OCFwK5rtN25HM5BckpxCeje65_0nAMillLPow8n2x9AYUilvNnFlW9X1trbaKhfvWhe-OqvjRvVhKudXcX9l4l8_iWoGF1bImJl_tdvBxcrp9qp1i9gPvjfN_ehOHTbMg5t5EL19cfKmeEnO1qdlcXRGdMqAEa40aC0qKiu2qYTe8CoRpgKWQaVZmovE5JzqmhsujK43uUoUN3XFBYWNgCw9iB5N3F3Xfrk2vsfGem2cU1vTXntMaCJlzmW6P0B3rfedqXHX2UZ1A1LAUTTiKBpHbThqw0k0MoYMg2jEIBr_iMYUAYs1JgiB-_DmAOW1cnWnttr6PTz00wxEiF1OsW_WmeGv7v9U_6t5eghgMoFt0P59D1bdZ8xEKji-Oz_FV_T843N4f4kX6W8NPa5M</recordid><startdate>199712</startdate><enddate>199712</enddate><creator>Cristallini, Caterina</creator><creator>Lazzeri, Luigi</creator><creator>Cascone, Maria Grazia</creator><creator>Polacco, Giovanni</creator><creator>Lupinacci, Domenico</creator><creator>Barbani, Niccoletta</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>199712</creationdate><title>Enzyme-based bioartificial polymeric materials: the α-amylase-poly(vinyl alcohol) system</title><author>Cristallini, Caterina ; Lazzeri, Luigi ; Cascone, Maria Grazia ; Polacco, Giovanni ; Lupinacci, Domenico ; Barbani, Niccoletta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3404-5ac0cc7b19b4db7cd5b27eb0460bc43872e851cf5e57ecfd8a2a5efb5710d7063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Applied sciences</topic><topic>bioartificial polymeric materials</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Calorimetry</topic><topic>delivery systems</topic><topic>Enzymes</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogels</topic><topic>Immobilization of enzymes and other molecules</topic><topic>Immobilization techniques</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular dynamics</topic><topic>Morphology</topic><topic>Organic polymers</topic><topic>Physicochemistry of polymers</topic><topic>poly(vinyl alcohol)</topic><topic>Polymer blends</topic><topic>Polyvinyl alcohols</topic><topic>Properties and characterization</topic><topic>Special properties (catalyst, reagent or carrier)</topic><topic>Thawing</topic><topic>Thermodynamic stability</topic><topic>X ray diffraction analysis</topic><topic>α-amylase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cristallini, Caterina</creatorcontrib><creatorcontrib>Lazzeri, Luigi</creatorcontrib><creatorcontrib>Cascone, Maria Grazia</creatorcontrib><creatorcontrib>Polacco, Giovanni</creatorcontrib><creatorcontrib>Lupinacci, Domenico</creatorcontrib><creatorcontrib>Barbani, Niccoletta</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Polymer international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cristallini, Caterina</au><au>Lazzeri, Luigi</au><au>Cascone, Maria Grazia</au><au>Polacco, Giovanni</au><au>Lupinacci, Domenico</au><au>Barbani, Niccoletta</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzyme-based bioartificial polymeric materials: the α-amylase-poly(vinyl alcohol) system</atitle><jtitle>Polymer international</jtitle><addtitle>Polym. Int</addtitle><date>1997-12</date><risdate>1997</risdate><volume>44</volume><issue>4</issue><spage>510</spage><epage>516</epage><pages>510-516</pages><issn>0959-8103</issn><eissn>1097-0126</eissn><abstract>The possibility of realizing bioartificial polymeric materials from blends of the enzyme α‐amylase (EC 3.2.1.1) and poly(vinyl alcohol), in the form of film and hydrogel, was investigated. The interactions at molecular level between the enzyme and the synthetic polymer have been studied by calorimetry, X‐ray diffractometry and morphological analysis. A certain degree of interaction through which the α‐amylase molecules influence the crystallinity of the poly(vinyl alcohol) was observed. The thermal stability of the enzyme in the blends was enhanced, while its biological activity did not vary significantly. In addition, hydrogels prepared by a freeze–thawing method can be considered suitable for manufacturing delivery systems capable of releasing enzymes which maintain their full biological activity. ©1997 SCI</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/(SICI)1097-0126(199712)44:4<510::AID-PI884>3.0.CO;2-0</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0959-8103 |
| ispartof | Polymer international, 1997-12, Vol.44 (4), p.510-516 |
| issn | 0959-8103 1097-0126 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_21299859 |
| source | Wiley |
| subjects | Applied sciences bioartificial polymeric materials Biological and medical sciences Biotechnology Calorimetry delivery systems Enzymes Exact sciences and technology Fundamental and applied biological sciences. Psychology Hydrogels Immobilization of enzymes and other molecules Immobilization techniques Methods. Procedures. Technologies Molecular dynamics Morphology Organic polymers Physicochemistry of polymers poly(vinyl alcohol) Polymer blends Polyvinyl alcohols Properties and characterization Special properties (catalyst, reagent or carrier) Thawing Thermodynamic stability X ray diffraction analysis α-amylase |
| title | Enzyme-based bioartificial polymeric materials: the α-amylase-poly(vinyl alcohol) system |
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