Structural characterization and immunological activity of polysaccharides from the tuber of Bletilla striata

• Published in: International journal of biological macromolecules 2019-02, Vol.122, p.628-635
• Main Authors: Wang, Yirui, Han, Shaowei, Li, Ruofei, Cui, Baosong, Ma, Xianjie, Qi, Xinzhu, Hou, Qi, Lin, Mingbao, Bai, Jinye, Li, Shuai
• Format: Article
• Language: English
• Subjects: Animals; Immunologic Factors - chemistry; Immunologic Factors - pharmacology; Leukocyte Count; Methylation; Mice; Molecular Weight; Monosaccharides - analysis; Orchidaceae - chemistry; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Tubers - chemistry; Polysaccharides - chemistry; Polysaccharides - pharmacology; Thymus Gland - immunology
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2018.10.201

Two water-soluble polysaccharides, BSP-1 and BSP-2, were extracted and purified from the tuber of Bletilla striata. The molecular weights of BSP-1 and BSP-2 were 83.54 kDa and 12.60 kDa, respectively. The composition and proportion of BSP-1 and BSP-2 were mannose and glucose in molar ratios of 4.0:1.0 and 3.0:1.0 respectively. The two polysaccharides had a linear backbone, consisting mainly of repeating β‑1,4‑linked d‑mannosyl residues and β‑1,4‑linked d‑glucosyl residues. BSP-1 exhibited immunomodulatory effects by increasing the thymus and spleen indices of immunocompromised model mice. •Two polysaccharides were isolated from the tuber of Bletilla striata.•The molecular weights of BSP-1, BSP-2 were 83.54 kDa, 12.60 kDa respectively.•The backbone consisted of 1,4‑linked mannosyl and 1,4‑linked glucosyl residues.•BSP-1 was shown to have immunomodulatory effects in vivo.