SnCl sub(2)-induced DNA damage and repair inhibition of MMS-caused lesions in V79 Chinese hamster fibroblasts

In order to clarify the molecular mechanisms of Sn super(2+) genotoxicity, we evaluated the induction of strand breaks, formamidopyrimidine DNA glycosylase (Fpg) and endonuclease III (Endo III) sensitive sites, and the interference with the repair of methyl methane sulfonate (MMS)-caused DNA damage...

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Bibliographic Details
Published in:Archives of toxicology 2009-08, Vol.83 (8), p.769-775
Main Authors: Viau, C M, Guecheva, Temenouga N, Sousa, F G, Pungartnik, C, Brendel, M, Saffi, J, Henriques, Joao Antonio Pegas
Format: Article
Language:English
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Summary:In order to clarify the molecular mechanisms of Sn super(2+) genotoxicity, we evaluated the induction of strand breaks, formamidopyrimidine DNA glycosylase (Fpg) and endonuclease III (Endo III) sensitive sites, and the interference with the repair of methyl methane sulfonate (MMS)-caused DNA damage in V79 Chinese hamster lung fibroblasts exposed to stannous chloride by comet assay. A concentration-related increase in the DNA damage induced by 2h SnCl sub(2) treatment at a concentration range of 50-1,000kM was observed (r=0.993; P
ISSN:0340-5761
1432-0738
DOI:10.1007/s00204-009-0409-z