Expression Pattern of Cdkn2b and Its Regulators in Canine Mammary Tumors

• Published in: Anticancer research 2018-11, Vol.38 (11), p.6333-6338
• Main Authors: Faro, Thamirys Aline Silva, DO Rosário Pinheiro, Danilo, Calcagno, Danielle Queiroz, Pereira, Washington Luiz Assunção, DE Aguirra, Lucien Roberta Valente Miranda, Burbano, Rommel Rodriguez, Harada, Maria Lúcia, DO Nascimento Borges, Bárbara
• Format: Article
• Language: English
• Subjects: Animals; Bisulfite; Breast cancer; Cancer; CDKN2B gene; Cyclin-dependent kinase; Cyclin-Dependent Kinase Inhibitor p15 - genetics; Cyclin-dependent kinases; Diagnostic systems; DNA Methylation; Dog Diseases - genetics; Dogs; Enzyme inhibitors; Epigenesis, Genetic; Female; Gene expression; Gene Expression Profiling - veterinary; Gene Expression Regulation, Neoplastic; Gene sequencing; Genes; Kinases; Mammary Neoplasms, Animal - genetics; Mitogen-Activated Protein Kinase 7 - genetics; Myc protein; Polymerase chain reaction; Proteins; Proto-Oncogene Proteins c-myc - genetics; Regulators; Sequence Analysis, DNA - veterinary; T-Box Domain Proteins - genetics; T-box gene; Tumors; Veterinary medicine
• ISSN: 0250-7005; 1791-7530
• DOI: 10.21873/anticanres.12991

In female dogs, mammary cancer is the most frequent cancer type, accounting for 50% of all tumors affecting these animals. Amongst the commonly altered genes in cancer is the cell-cycle regulator cyclin-dependent kinase inhibitor 2B (Cdkn2b), whose expression is negatively regulated by protein products of BMI1 proto-oncogene (Bmi1), MYC proto-oncogene (Myc) and T-box gene transcription factor 2 (Tbx2) genes. Considering this, the aim of this study was to evaluate the expression pattern of the Cdkn2b gene and these regulators in canine mammary tumors of dogs from Northern Brazil (Belém, Pará). Gene expression in samples from 33 animals was assessed by real-time polymerase chain reaction. To check the influence of methylation on gene expression, bisulfite sequencing polymerase chain reaction was also performed. All studied genes, except Cdkn2b, were found at increased expression levels in tumor tissue when compared with control samples. No correlation between expression and methylation data was observed. Our results suggest these markers may have a diagnostic value in the veterinary clinic.