Iron Sources Used by the Nonpathogenic Lactic Acid Bacterium Lactobacillus sakei as Revealed by Electron Energy Loss Spectroscopy and Secondary-Ion Mass Spectrometry

Lactobacillus sakei is a lactic acid bacterium naturally found on meat. Although it is generally acknowledged that lactic acid bacteria are rare species in the microbial world which do not have iron requirements, the genome sequence of L. sakei 23K has revealed quite complete genetic equipment dedic...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2010, Vol.76 (2), p.560-565
Main Authors: Duhutrel, Philippe, Bordat, Christian, Wu, Ting-Di, Zagorec, Monique, Guerquin-Kern, Jean-Luc, Champomier-Vergès, Marie-Christine
Format: Article
Language:English
Subjects:
Bacteria
Bacteriology
Biological and medical sciences
Catalase - metabolism
Cells
Energy
Fundamental and applied biological sciences. Psychology
Genomics
Gram-positive bacteria
Heme - metabolism
Iron
Iron - metabolism
Lactobacillus - metabolism
Life Sciences
Mass spectrometry
Meat - microbiology
Microbiology
Microbiology and Parasitology
Physiology
Spectrometry, Mass, Secondary Ion - methods
Spectroscopy, Electron Energy-Loss - methods
Spectrum analysis
Transferrin - metabolism
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Summary:Lactobacillus sakei is a lactic acid bacterium naturally found on meat. Although it is generally acknowledged that lactic acid bacteria are rare species in the microbial world which do not have iron requirements, the genome sequence of L. sakei 23K has revealed quite complete genetic equipment dedicated to transport and use of this metal. Here, we aimed to investigate which iron sources could be used by this species as well as their role in the bacterium's physiology. Therefore, we developed a microscopy approach based on electron energy loss spectroscopy (EELS) analysis and nano-scale secondary-ion mass spectrometry (SIMS) in order to analyze the iron content of L. sakei cells. This revealed that L. sakei can use iron sources found in its natural ecosystem, myoglobin, hemoglobin, hematin, and transferrin, to ensure long-term survival during stationary phase. This study reveals that analytical image methods (EELS and SIMS) are powerful complementary tools for investigation of metal utilization by bacteria.
ISSN:0099-2240
1098-5336
1098-6596
DOI:10.1128/AEM.02205-09