Proteomics of proteins associated with astaxanthin accumulation in the green algae Haematococcus lacustris under the influence of sodium orthovanadate

An extensive proteomics analysis has identified proteins associated with astaxanthin accumulation in the green algae Haematococcus lacustris under oxidative stress induced by sodium orthovanadate (SOV). Measurement of total carotenoid accumulation per cell biomass showed an increase from 81 to 136 p...

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Bibliographic Details
Published in:Biotechnology letters 2009-12, Vol.31 (12), p.1917-1922
Main Authors: Tran, Ngoc-Phuong, Park, Jae-Kweon, Hong, Seong-Joo, Lee, Choul-Gyun
Format: Article
Language:English
Subjects:
Accumulation
Algae
Algal Proteins - analysis
Applied Microbiology
Aquatic plants
Astaxanthin
Biochemistry
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Carotenoids
Carotenoids - analysis
Down-Regulation
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling
Haematococcus lacustris
Life Sciences
Microbiology
Original Research Paper
Oxidants - toxicity
Oxidation
Oxidative stress
Proteins
Proteome - analysis
Proteomics
Sodium
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Stresses
Up-Regulation
Vanadates - toxicity
Volvocida - chemistry
Volvocida - drug effects
Volvocida - metabolism
Xanthophylls - metabolism
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Summary:An extensive proteomics analysis has identified proteins associated with astaxanthin accumulation in the green algae Haematococcus lacustris under oxidative stress induced by sodium orthovanadate (SOV). Measurement of total carotenoid accumulation per cell biomass showed an increase from 81 to 136 pg/cell after being exposed to 2.5 mM SOV, when compared to the control cells at day 3 of cultivation. A total of 83 proteins were differentially expressed in SOV-treated H. lacustris in comparison with control cells. They consisted of 34 down-regulated and 49 up-regulated proteins. Of these, 17 highly-expressed proteins were analyzed by MALDI-TOF-MS to identify the function of the differentially expressed proteins in response to oxidative stress in H. lacustris.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-009-0095-1