Could chlorhexidine be an adequate positive control for antimicrobial photodynamic therapy in- in vitro studies?

•Chlorhexidine (CHX) diluted in a neutralizer did not present substantivity.•CHX + neutralizer and aPDT presented similar antimicrobial immediate effects.•It is advocate the use of CHX + neutralizer as a positive control to aPDT. Background: Chlorhexidine digluconate (CHX) is commonly applied as pos...

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Published in:Photodiagnosis and photodynamic therapy 2019-03, Vol.25, p.58-62
Main Authors: Lamarque, Giuliana Campos Chaves, Méndez, Daniela Alejandra Cusicanqui, Gutierrez, Eliezer, Dionisio, Evandro José, Machado, Maria Aparecida Andrade Moreira, Oliveira, Thaís Marchini, Rios, Daniela, Cruvinel, Thiago
Format: Article
Language:English
Subjects:
Antimicrobial photodynamic therapy
Biofilms - drug effects
Child
Chlorhexidine
Chlorhexidine - analogs & derivatives
Chlorhexidine - pharmacology
Curcumin - pharmacology
Dental caries
Dental Caries - microbiology
Dentin - drug effects
Dose-Response Relationship, Drug
Humans
Lactobacillus - drug effects
Microbial Viability - drug effects
Oral biofilms
Photochemotherapy
Photochemotherapy - methods
Photosensitizing Agents - pharmacology
Research Design
Stem Cells
Streptococcus mutans - drug effects
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Summary:•Chlorhexidine (CHX) diluted in a neutralizer did not present substantivity.•CHX + neutralizer and aPDT presented similar antimicrobial immediate effects.•It is advocate the use of CHX + neutralizer as a positive control to aPDT. Background: Chlorhexidine digluconate (CHX) is commonly applied as positive control of new antimicrobials, because it is considered the gold-standard for chemical plaque control. The aim of this study was to compare the effect of treatments with curcumin-mediated aPDT and CHX in relation to the viability of specific microorganism groups in two distinct times (immediately and 24 h later). Methods: Dentin caries microcosms were grown on bovine dentin discs (37 °C, anaerobiosis) for 3 days in the Active Attachment Amsterdam Biofilm Model. The biofilms were treated with 300 μM curcumin and 75 J.cm−² LED, or 0.06% and 0.12% CHX. Then, total microorganisms, total streptococci, mutans streptococci, and total lactobacilli counts were determined. The statistical analysis was conducted by Kruskal-Wallis and post-hoc Dunn's tests (P 
ISSN:1572-1000
1873-1597
DOI:10.1016/j.pdpdt.2018.11.004