Relating the microstructure of enzyme dispersions in organic solvents to their kinetic behavior

The effect of ultrasound irradiation on activity and particle size of a subtilisin-catalyzed interesterification reaction in an organic solvent was studied. Across a range of levels of water addition and irradiation amplitude, sonication failed to increase reaction rate compared with stirred systems...

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Bibliographic Details
Published in:Enzyme and microbial technology 1998-02, Vol.22 (3), p.147-151
Main Authors: Bracey, Emma, Stenning, Robert A., Brooker, Brian E.
Format: Article
Language:English
Subjects:
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Electron microscopy
Enzyme inhibition
Esterification
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Microstructure
organic solvent
Organic solvents
particle size
Particle size analysis
Reaction kinetics
Subtilisin
Ultrasonic effects
ultrasound irradiation
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Summary:The effect of ultrasound irradiation on activity and particle size of a subtilisin-catalyzed interesterification reaction in an organic solvent was studied. Across a range of levels of water addition and irradiation amplitude, sonication failed to increase reaction rate compared with stirred systems. Sonication was shown to be effective in reducing particle size, but the effect of any reduction in diffusional limitations on reaction rate was exceeded by an inhibitory effect of the treatment. This inhibition was found to be irreversible upon cessation of sonication and aqueous assay of activity, or recycling of the enzyme into a stirred reaction system.
ISSN:0141-0229
1879-0909
DOI:10.1016/S0141-0229(97)00138-5