Excelsanone, a new isoflavonoid from Erythrina excelsa (Fabaceae), with in vitro antioxidant and in vitro cytotoxic effects on prostate cancer cells lines

• Published in: Natural product research 2020-03, Vol.34 (5), p.659-667
• Main Authors: Gbaweng, Abel Joël Yaya, Daïrou, Hadidjatou, Zingué, Stephane, Emmanuel, Talla, Tchinda, Alembert Tiabou, Frédérich, Michel, Mbafor, Joseph Tanyi
• Format: Article
• Language: English
• Subjects: Acetic acid; Antineoplastic Agents - chemistry; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; antioxidant; Antioxidants; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Bark; Biotechnology; Catechin; Cell Line, Tumor; Cell Proliferation - drug effects; Cytotoxicity; Erythrina; Erythrina - chemistry; Erythrina excelsa; Ethyl acetate; Fabaceae; Fabaceae - chemistry; Free radicals; Human health sciences; Humans; Isoflavones - chemistry; Isoflavones - isolation & purification; Isoflavones - pharmacology; isoflavonoid; Male; NMR; Nuclear magnetic resonance; Pharmacie, pharmacologie & toxicologie; Pharmacy, pharmacology & toxicology; Plant Extracts - chemistry; Plant Extracts - pharmacology; Prostate cancer; Prostatic Neoplasms - drug therapy; Prostatic Neoplasms - pathology; Scavenging; Sciences de la santé humaine; Toxicity testing; Tumor cell lines
• ISSN: 1478-6419; 1478-6427; 1478-6427
• DOI: 10.1080/14786419.2018.1495639

A new isoflavonoid, excelsanone (2), was isolated from the ethyl acetate extract of Erythrina excelsa stem bark, together with three known compounds namely 6,8-diprenylgenistein (3), β-sitosterol (1) and sitosteryl-β-D-glucopyranoside (4). Their structures were elucidated using spectroscopic methods (HR-ESI-MS, NMR and IR) and by comparison with some literature data. The antioxidant activity of crude extracts and two isolated compounds was evaluated using free radical scavenging (DPPH) and Ferric Reducing Ability Power (FRAP) methods with catechin as standard. The results of the radical scavenging activity showed that excelsanone (2) has a moderate potential with an IC 50 of 1.31 mg/ml. The cytotoxicity of compounds 2 and 3 as well as the ethyl acetate extract was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in two prostate cancer cell lines (DU145 and PC3). Excelsanone (2) induced a greater cytotoxicity in all tested cell lines, with a significant inhibition of DU145 cells growth in a concentration-dependent manner.