Role of extracellular calcium ions at early stages of human T-lymphocyte polyclonal activation in vitro

• Published in: Biochemistry (Moscow) 2009-03, Vol.74 (3), p.268-275
• Main Authors: Litvinov, I. S., Fomichev, M. A., Kirpichnikov, M. P.
• Format: Article
• Language: English
• Subjects: Antigens, CD - immunology; Antigens, Differentiation, T-Lymphocyte - immunology; Biochemistry; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; Blood; Calcium; Calcium - metabolism; Calcium - physiology; CD3 Complex - immunology; Cells, Cultured; Chelating Agents - pharmacology; Cytokines; Dose-Response Relationship, Drug; Egtazic Acid - pharmacology; Extracellular Space - metabolism; Humans; Interferon; Interferon-gamma - immunology; Interleukin-2 - immunology; Interleukin-2 Receptor alpha Subunit - immunology; Ionomycin - pharmacology; Ionophores - pharmacology; Lectins, C-Type; Life Sciences; Lymphocyte Activation - drug effects; Lymphocyte Activation - immunology; Lymphocytes; Microbiology; T cell receptors; T-Lymphocytes - cytology; T-Lymphocytes - drug effects; T-Lymphocytes - immunology; Tetradecanoylphorbol Acetate - pharmacology; Time Factors
• ISSN: 0006-2979; 1608-3040
• DOI: 10.1134/S0006297909030043

In this work we comparatively analyzed interleukin-2 (IL-2) and interferon γ production (IFN-γ) and also CD69 and CD25 expression by activated T-cells depending on extracellular calcium concentration ([Ca 2+ ] e ), which was varied with EGTA. The expression of CD69 molecules on the surface of T-cells depended only on the presence of phorbol myristate acetate, occurred at [Ca 2+ ] e higher than 0.2 mM, and did not require the presence of ionomycin. The increase in [Ca 2+ ] e by itself cannot induce expression of CD25 and CD69 molecules by activated cells. The values of [Ca 2+ ] e , at which maximal fractions of CD3 + CD69 + (IL-2) + , CD3 + CD69 + (IFN-γ) + , and CD3 + CD25 + activated T-cells were reached, never coincided with mean values of [Ca 2+ ] e for healthy donors and were different from each other. So, there is different [Ca 2+ ] e dependence for initial stages of activated T-cells differentiation. The relation between T-cells activation parameters and their differentiation is discussed.