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Role of extracellular calcium ions at early stages of human T-lymphocyte polyclonal activation in vitro
In this work we comparatively analyzed interleukin-2 (IL-2) and interferon γ production (IFN-γ) and also CD69 and CD25 expression by activated T-cells depending on extracellular calcium concentration ([Ca 2+ ] e ), which was varied with EGTA. The expression of CD69 molecules on the surface of T-cell...
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| Published in: | Biochemistry (Moscow) 2009-03, Vol.74 (3), p.268-275 |
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| Main Authors: | , , |
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| Language: | English |
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| container_end_page | 275 |
| container_issue | 3 |
| container_start_page | 268 |
| container_title | Biochemistry (Moscow) |
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| creator | Litvinov, I. S. Fomichev, M. A. Kirpichnikov, M. P. |
| description | In this work we comparatively analyzed interleukin-2 (IL-2) and interferon γ production (IFN-γ) and also CD69 and CD25 expression by activated T-cells depending on extracellular calcium concentration ([Ca
2+
]
e
), which was varied with EGTA. The expression of CD69 molecules on the surface of T-cells depended only on the presence of phorbol myristate acetate, occurred at [Ca
2+
]
e
higher than 0.2 mM, and did not require the presence of ionomycin. The increase in [Ca
2+
]
e
by itself cannot induce expression of CD25 and CD69 molecules by activated cells. The values of [Ca
2+
]
e
, at which maximal fractions of CD3
+
CD69
+
(IL-2)
+
, CD3
+
CD69
+
(IFN-γ)
+
, and CD3
+
CD25
+
activated T-cells were reached, never coincided with mean values of [Ca
2+
]
e
for healthy donors and were different from each other. So, there is different [Ca
2+
]
e
dependence for initial stages of activated T-cells differentiation. The relation between T-cells activation parameters and their differentiation is discussed. |
| doi_str_mv | 10.1134/S0006297909030043 |
| format | article |
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2+
]
e
), which was varied with EGTA. The expression of CD69 molecules on the surface of T-cells depended only on the presence of phorbol myristate acetate, occurred at [Ca
2+
]
e
higher than 0.2 mM, and did not require the presence of ionomycin. The increase in [Ca
2+
]
e
by itself cannot induce expression of CD25 and CD69 molecules by activated cells. The values of [Ca
2+
]
e
, at which maximal fractions of CD3
+
CD69
+
(IL-2)
+
, CD3
+
CD69
+
(IFN-γ)
+
, and CD3
+
CD25
+
activated T-cells were reached, never coincided with mean values of [Ca
2+
]
e
for healthy donors and were different from each other. So, there is different [Ca
2+
]
e
dependence for initial stages of activated T-cells differentiation. The relation between T-cells activation parameters and their differentiation is discussed.</description><identifier>ISSN: 0006-2979</identifier><identifier>EISSN: 1608-3040</identifier><identifier>DOI: 10.1134/S0006297909030043</identifier><identifier>PMID: 19364320</identifier><language>eng</language><publisher>Dordrecht: SP MAIK Nauka/Interperiodica</publisher><subject>Antigens, CD - immunology ; Antigens, Differentiation, T-Lymphocyte - immunology ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Bioorganic Chemistry ; Blood ; Calcium ; Calcium - metabolism ; Calcium - physiology ; CD3 Complex - immunology ; Cells, Cultured ; Chelating Agents - pharmacology ; Cytokines ; Dose-Response Relationship, Drug ; Egtazic Acid - pharmacology ; Extracellular Space - metabolism ; Humans ; Interferon ; Interferon-gamma - immunology ; Interleukin-2 - immunology ; Interleukin-2 Receptor alpha Subunit - immunology ; Ionomycin - pharmacology ; Ionophores - pharmacology ; Lectins, C-Type ; Life Sciences ; Lymphocyte Activation - drug effects ; Lymphocyte Activation - immunology ; Lymphocytes ; Microbiology ; T cell receptors ; T-Lymphocytes - cytology ; T-Lymphocytes - drug effects ; T-Lymphocytes - immunology ; Tetradecanoylphorbol Acetate - pharmacology ; Time Factors</subject><ispartof>Biochemistry (Moscow), 2009-03, Vol.74 (3), p.268-275</ispartof><rights>Pleiades Publishing, Ltd. 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c352t-8ed427cddb776a5bdd4f6ef50790a053bf1f2b18f57b3105232e998a6644988c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19364320$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Litvinov, I. S.</creatorcontrib><creatorcontrib>Fomichev, M. A.</creatorcontrib><creatorcontrib>Kirpichnikov, M. P.</creatorcontrib><title>Role of extracellular calcium ions at early stages of human T-lymphocyte polyclonal activation in vitro</title><title>Biochemistry (Moscow)</title><addtitle>Biochemistry Moscow</addtitle><addtitle>Biochemistry (Mosc)</addtitle><description>In this work we comparatively analyzed interleukin-2 (IL-2) and interferon γ production (IFN-γ) and also CD69 and CD25 expression by activated T-cells depending on extracellular calcium concentration ([Ca
2+
]
e
), which was varied with EGTA. The expression of CD69 molecules on the surface of T-cells depended only on the presence of phorbol myristate acetate, occurred at [Ca
2+
]
e
higher than 0.2 mM, and did not require the presence of ionomycin. The increase in [Ca
2+
]
e
by itself cannot induce expression of CD25 and CD69 molecules by activated cells. The values of [Ca
2+
]
e
, at which maximal fractions of CD3
+
CD69
+
(IL-2)
+
, CD3
+
CD69
+
(IFN-γ)
+
, and CD3
+
CD25
+
activated T-cells were reached, never coincided with mean values of [Ca
2+
]
e
for healthy donors and were different from each other. So, there is different [Ca
2+
]
e
dependence for initial stages of activated T-cells differentiation. The relation between T-cells activation parameters and their differentiation is discussed.</description><subject>Antigens, CD - immunology</subject><subject>Antigens, Differentiation, T-Lymphocyte - immunology</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bioorganic Chemistry</subject><subject>Blood</subject><subject>Calcium</subject><subject>Calcium - metabolism</subject><subject>Calcium - physiology</subject><subject>CD3 Complex - immunology</subject><subject>Cells, Cultured</subject><subject>Chelating Agents - pharmacology</subject><subject>Cytokines</subject><subject>Dose-Response Relationship, Drug</subject><subject>Egtazic Acid - pharmacology</subject><subject>Extracellular Space - metabolism</subject><subject>Humans</subject><subject>Interferon</subject><subject>Interferon-gamma - immunology</subject><subject>Interleukin-2 - immunology</subject><subject>Interleukin-2 Receptor alpha Subunit - immunology</subject><subject>Ionomycin - pharmacology</subject><subject>Ionophores - pharmacology</subject><subject>Lectins, C-Type</subject><subject>Life Sciences</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Lymphocyte Activation - immunology</subject><subject>Lymphocytes</subject><subject>Microbiology</subject><subject>T cell receptors</subject><subject>T-Lymphocytes - cytology</subject><subject>T-Lymphocytes - drug effects</subject><subject>T-Lymphocytes - immunology</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><subject>Time Factors</subject><issn>0006-2979</issn><issn>1608-3040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNp1kUtLxDAUhYMoOj5-gBsJLtxVbx59ZCmDLxgQdFyXNE1nKmkzJqnYf2_KDAiKq3A53zm5D4TOCVwTwvjNKwBkVOQCBDAAzvbQjGRQJAw47KPZJCeTfoSOvX-PJQXBDtERESzjjMIMrV6s0dg2WH8FJ5U2ZjDSYSWNaocOt7b3WAaspTMj9kGutJ_o9dDJHi8TM3abtVVj0HhjzaiM7aXBUoX2U4Zoxm2PP9vg7Ck6aKTx-mz3nqC3-7vl_DFZPD88zW8XiWIpDUmha05zVddVnmcyreqaN5luUogzSkhZ1ZCGVqRo0rxiBFLKqBaikFnGuSgKxU7Q1TZ34-zHoH0ou9ZPY8le28GXlDCSFymJ4OUv8N0OLnYfGUaZKEDwCJEtpJz13umm3Li2k24sCZTTCco_J4iei13wUHW6_nHsdh4BugV8lPqVdj8__5_6DT2JkGU</recordid><startdate>20090301</startdate><enddate>20090301</enddate><creator>Litvinov, I. 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S. ; Fomichev, M. A. ; Kirpichnikov, M. P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-8ed427cddb776a5bdd4f6ef50790a053bf1f2b18f57b3105232e998a6644988c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Antigens, CD - immunology</topic><topic>Antigens, Differentiation, T-Lymphocyte - immunology</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bioorganic Chemistry</topic><topic>Blood</topic><topic>Calcium</topic><topic>Calcium - metabolism</topic><topic>Calcium - physiology</topic><topic>CD3 Complex - immunology</topic><topic>Cells, Cultured</topic><topic>Chelating Agents - pharmacology</topic><topic>Cytokines</topic><topic>Dose-Response Relationship, Drug</topic><topic>Egtazic Acid - pharmacology</topic><topic>Extracellular Space - metabolism</topic><topic>Humans</topic><topic>Interferon</topic><topic>Interferon-gamma - immunology</topic><topic>Interleukin-2 - immunology</topic><topic>Interleukin-2 Receptor alpha Subunit - immunology</topic><topic>Ionomycin - pharmacology</topic><topic>Ionophores - pharmacology</topic><topic>Lectins, C-Type</topic><topic>Life Sciences</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Lymphocyte Activation - immunology</topic><topic>Lymphocytes</topic><topic>Microbiology</topic><topic>T cell receptors</topic><topic>T-Lymphocytes - cytology</topic><topic>T-Lymphocytes - drug effects</topic><topic>T-Lymphocytes - immunology</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Litvinov, I. S.</creatorcontrib><creatorcontrib>Fomichev, M. A.</creatorcontrib><creatorcontrib>Kirpichnikov, M. 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S.</au><au>Fomichev, M. A.</au><au>Kirpichnikov, M. P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of extracellular calcium ions at early stages of human T-lymphocyte polyclonal activation in vitro</atitle><jtitle>Biochemistry (Moscow)</jtitle><stitle>Biochemistry Moscow</stitle><addtitle>Biochemistry (Mosc)</addtitle><date>2009-03-01</date><risdate>2009</risdate><volume>74</volume><issue>3</issue><spage>268</spage><epage>275</epage><pages>268-275</pages><issn>0006-2979</issn><eissn>1608-3040</eissn><abstract>In this work we comparatively analyzed interleukin-2 (IL-2) and interferon γ production (IFN-γ) and also CD69 and CD25 expression by activated T-cells depending on extracellular calcium concentration ([Ca
2+
]
e
), which was varied with EGTA. The expression of CD69 molecules on the surface of T-cells depended only on the presence of phorbol myristate acetate, occurred at [Ca
2+
]
e
higher than 0.2 mM, and did not require the presence of ionomycin. The increase in [Ca
2+
]
e
by itself cannot induce expression of CD25 and CD69 molecules by activated cells. The values of [Ca
2+
]
e
, at which maximal fractions of CD3
+
CD69
+
(IL-2)
+
, CD3
+
CD69
+
(IFN-γ)
+
, and CD3
+
CD25
+
activated T-cells were reached, never coincided with mean values of [Ca
2+
]
e
for healthy donors and were different from each other. So, there is different [Ca
2+
]
e
dependence for initial stages of activated T-cells differentiation. The relation between T-cells activation parameters and their differentiation is discussed.</abstract><cop>Dordrecht</cop><pub>SP MAIK Nauka/Interperiodica</pub><pmid>19364320</pmid><doi>10.1134/S0006297909030043</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-2979 |
| ispartof | Biochemistry (Moscow), 2009-03, Vol.74 (3), p.268-275 |
| issn | 0006-2979 1608-3040 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_21317851 |
| source | Springer Nature |
| subjects | Antigens, CD - immunology Antigens, Differentiation, T-Lymphocyte - immunology Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Blood Calcium Calcium - metabolism Calcium - physiology CD3 Complex - immunology Cells, Cultured Chelating Agents - pharmacology Cytokines Dose-Response Relationship, Drug Egtazic Acid - pharmacology Extracellular Space - metabolism Humans Interferon Interferon-gamma - immunology Interleukin-2 - immunology Interleukin-2 Receptor alpha Subunit - immunology Ionomycin - pharmacology Ionophores - pharmacology Lectins, C-Type Life Sciences Lymphocyte Activation - drug effects Lymphocyte Activation - immunology Lymphocytes Microbiology T cell receptors T-Lymphocytes - cytology T-Lymphocytes - drug effects T-Lymphocytes - immunology Tetradecanoylphorbol Acetate - pharmacology Time Factors |
| title | Role of extracellular calcium ions at early stages of human T-lymphocyte polyclonal activation in vitro |
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