Dm nxf1/sbr gene affects the formation of meiotic spindle in female Drosophila melanogaster

• Published in: Chromosome research 2009-10, Vol.17 (7), p.833-845
• Main Authors: Golubkova, Elena V., Markova, Ekaterina G., Markov, Anton V., Avanesyan, Elina O., Nokkala, Seppo, Mamon, Ludmila A.
• Format: Article
• Language: English
• Subjects: Alleles; Animal Genetics and Genomics; Animals; Base Sequence; Biomedical and Life Sciences; Cell Biology; Drosophila melanogaster; Drosophila melanogaster - chemistry; Drosophila melanogaster - cytology; Drosophila melanogaster - genetics; Drosophila melanogaster - metabolism; Drosophila Proteins - chemistry; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; Female; Gene Deletion; Genes; Human Genetics; Insects; Life Sciences; Male; Meiosis; Models, Molecular; Molecular Sequence Data; Mutation; Nuclear Proteins - chemistry; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Plant Genetics and Genomics; Protein Structure, Secondary; Protein Structure, Tertiary; Proteins; Ribonucleic acid; RNA; RNA-Binding Proteins - chemistry; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; Sequence Alignment; Spindle Apparatus - genetics; Studies
• ISSN: 0967-3849; 1573-6849
• DOI: 10.1007/s10577-009-9046-x

The small bristles ( sbr ) gene of Drosophila melanogaster belongs to the family of nuclear export factor ( NXF ) genes that participate in mRNA nuclear export. During meiosis, females of Drosophila melanogaster that carry various combinations of mutant alleles of the Dm nxf1 / sbr gene exhibit disruption of the division spindle and misalignment of chromosomes at the metaphase plate. Meiosis of sbr 5 /+ females is characterized by the formation of tripolar spindles during the first cell division. According to the sequencing results, the sbr 5 (l(1)K4) lethal allele is a deletion of 492 nucleotides. In SBR 5 protein, 57 of the 146 amino acids that have been lost by deletion belong to the NTF2-like domain.