Genetic diversity of rhinoviruses detected at a tertiary hospital in Catalonia (Spain) during the 2014-2017 seasons

To describe the genetic diversity of rhinovirus (RV) from patients attended at a tertiary hospital in Barcelona (Spain) from October 2014 to May 2017. RV detection was performed by real-time multiplex RT-PCR. A specific real-time quantitive retrotranscription PCR (qRT-PCR) was carried out to select...

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Bibliographic Details
Published in:Future microbiology 2018-11, Vol.13 (14), p.1565-1573
Main Authors: Andrés, Cristina, Peremiquel-Trillas, Paula, Gimferrer, Laura, Isern, Anna, Piñana, Maria, Rodrigo-Pendás, José Ángel, Codina, Maria Gema, Martín, María del Carmen, Fuentes, Francisco, Rubio, Susana, Campins-Martí, Magda, Pumarola, Tomàs, Antón, Andrés
Format: Article
Language:English
Subjects:
Adolescent
Adult
Child
Child, Preschool
Emergency medical care
evolution
Female
Genetic diversity
Genetic Variation - genetics
Humans
Infant
Infections
Influenza
Laboratories
Male
Middle Aged
mild respiratory diseases
molecular epidemiology
Odds Ratio
Patients
pediatric patients
Phylogenetics
Phylogeny
Picornaviridae Infections - epidemiology
Picornaviridae Infections - virology
Polymerase chain reaction
respiratory tract infection
Respiratory Tract Infections - epidemiology
Respiratory Tract Infections - virology
respiratory virus
Retrospective Studies
Rhinovirus
Rhinovirus - genetics
Rhinovirus - isolation & purification
RV circulation
Seasons
Software
Spain - epidemiology
Statistical analysis
Statistics, Nonparametric
surveillance
Tertiary Care Centers
Viral Matrix Proteins - genetics
Viruses
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Summary:To describe the genetic diversity of rhinovirus (RV) from patients attended at a tertiary hospital in Barcelona (Spain) from October 2014 to May 2017. RV detection was performed by real-time multiplex RT-PCR. A specific real-time quantitive retrotranscription PCR (qRT-PCR) was carried out to select those samples (Ct < 35) for molecular characterization based on partial VP4/2 protein. Phylogenetic characterization revealed proportions of 63% RV-A, 6% RV-B and 31% RV-C (119 different types). RV-A circulated throughout all the study period, with a minor circulation during winter, just when RV-C prevailed. Differences between age medians by RV-specie were reported. The large genetic diversity of RV detected in our area is described here. The variable cocirculation of multiple RV types is also reported, showing differences by age.
ISSN:1746-0913
1746-0921
DOI:10.2217/fmb-2018-0151