Extracellular DNA in the Dynamics of Uncomplicated Pregnancy

• Published in: Bulletin of experimental biology and medicine 2018-11, Vol.166 (1), p.92-95
• Main Authors: Karapetyan, А. О., Baev, О. R., Krasnyi, А. М., Sadekova, А. А., Мullabaeva, S. М.
• Format: Article
• Language: English
• Subjects: Adult; Biomedical and Life Sciences; Biomedicine; Cell Biology; Deoxyribonucleic acid; DNA; DNA - genetics; DNA Methylation - genetics; Experimental Methods for Clinic; Female; Fetuses; Gestation; Gestational Age; Humans; Internal Medicine; Laboratory Medicine; Pathology; Placenta; Placenta - metabolism; Polymerase Chain Reaction; Pregnancy; Pregnancy complications; Pregnancy Trimester, Second; Pregnancy Trimester, Third; Tumor Suppressor Proteins - genetics
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-018-4295-3

We analyzed the concentration of extracellular DNA and its fractions in the dynamics of uncomplicated pregnancy Thirty women with singleton pregnancy were examined. The concentration of total, maternal, and fetal cell-free DNA in maternal blood was measured at gestation weeks 11-14, 24-26, and 30-32. The level of total cell-free DNA was evaluated by measuring the concentration of RASSF1A gene using quantitative PCR analysis, the level of cell-free fetal DNA was assessed by determining the hypermethylated part of RASSF1A gene. The concentration of total cell-free DNA and cell-free maternal DNA did not change during the first half of pregnancy, but increased after 24-26 weeks. The level of cell-free fetal DNA increased from the first to the second and third trimester: 14.15 (2.32-36.25), 24.87 (6.29-129.32), and 32.62 (8.97-133.52) GE/ml ( p