Complete genome sequence of a putative novel potyvirus isolated from Platycodon grandiflorum

The complete 9,556-nt genomic sequence of a putative new potyvirus, tentatively named “platycodon mild mottle virus” (PlaMMV), infecting Platycodon grandiflorum was determined (GenBank accession no. MH779625). A single large open reading frame (ORF; nt 121-9360, 3079 aa) encoding a polyprotein that...

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Bibliographic Details
Published in:Archives of virology 2019-02, Vol.164 (2), p.621-624
Main Authors: Lim, Seungmo, Lee, Su-Heon, Moon, Jae Sun
Format: Article
Language:English
Subjects:
Amino acid sequence
Amino acids
Annotated Sequence Record
Base Sequence
Biomedical and Life Sciences
Biomedicine
Comparative analysis
Genome, Viral
Genomes
Infectious Diseases
Medical Microbiology
Nucleotide sequence
Open Reading Frames
Phylogenetics
Phylogeny
Plant Diseases - virology
Plant viruses
Platycodon - virology
Platycodon grandiflorum
Potyvirus - classification
Potyvirus - genetics
Potyvirus - isolation & purification
Proteins
Viral infections
Viral Proteins - genetics
Virology
Viruses
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Summary:The complete 9,556-nt genomic sequence of a putative new potyvirus, tentatively named “platycodon mild mottle virus” (PlaMMV), infecting Platycodon grandiflorum was determined (GenBank accession no. MH779625). A single large open reading frame (ORF; nt 121-9360, 3079 aa) encoding a polyprotein that was predicted to be cleaved into 10 mature proteins was detected. By comparison with other potyviruses, nine cleavage sites and conserved domains/motifs were identified. PlaMMV also has a highly conserved GA 6 motif (nt 2857-2863) that could enable a small overlapping ORF (PIPO) embedded within the P3 cistron to be translated by a viral polymerase slippage mechanism. A BLAST analysis showed that the complete PlaMMV nucleotide sequence shared 70% identity (40% query coverage) with carrot thin leaf virus (JX156434) and that the complete amino acid sequence of the PlaMMV polyprotein shared 54% identity (98% query coverage) with Thunberg fritillary mosaic virus (CAI59123). These results suggest that PlaMMV is a new member of the genus Potyvirus in the family Potyviridae .
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-018-4078-1