Antimicrobial agent isolated from Coptidis rhizome extract incubated with Rhodococcus sp. strain BD7100

Coptidis rhizome (CR) is a widely used herbal medicine that contains protoberberine-type alkaloids. CR extract exhibits various pharmacologic activities. A previous study reported the isolation of Rhodococcus sp. strain BD7100 as a berberine (BBR)-utilizing bacterium, and the BBR-degradation pathway...

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Bibliographic Details
Published in:Journal of antibiotics 2019-02, Vol.72 (2), p.71-78
Main Authors: Ishikawa, Kazuki, Takahashi, Kazunori, Hosoi, Syun, Takeda, Hisashi, Yoshida, Hinaka, Wakana, Daigo, Tsubuki, Masayoshi, Sato, Fumihiko, Tojo, Motoaki, Hosoe, Tomoo
Format: Article
Language:English
Subjects:
101/58
639/638/403/936
639/638/403/977
Antibacterial agents
Antimicrobial agents
Aquatic plants
Bacteriology
Biomedical and Life Sciences
Bioorganic Chemistry
Herbal medicine
Life Sciences
Medicinal Chemistry
Microbiology
Organic Chemistry
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Summary:Coptidis rhizome (CR) is a widely used herbal medicine that contains protoberberine-type alkaloids. CR extract exhibits various pharmacologic activities. A previous study reported the isolation of Rhodococcus sp. strain BD7100 as a berberine (BBR)-utilizing bacterium, and the BBR-degradation pathway has been investigated. When we incubated strain BD7100 cells with CR extract, the number of viable cells declined with the degradation of components in the CR extract, and the culture broth exhibited antibacterial activity against strain BD7100. These results suggest that CR extract cultured in the presence of strain BD7100 contains one or more antibacterial agents. In this study, we isolated coptirhoquinone A ( 1 ) from CR extract incubated with strain BD7100 in Luria–Bertani (LB) medium, and the structure was elucidated using NMR and MS analysis. We also report the total synthesis and antimicrobial activities of 1 against bacteria, fungi, and Pythium sp.
ISSN:0021-8820
1881-1469
DOI:10.1038/s41429-018-0114-3