Application of SPME/GC-MS To Characterize Metabolites in the Biodesulfurization of Organosulfur Model Compounds in Bitumen

A combined solid-phase microextraction/GC-MS analytical technique was used to monitor the formation of metabolites in the biodesulfurization of the bitumen model organosulfur compounds, dibenzothiophene (DBT) and the dialkylated derivative 4,6-diethyldibenzothiophene (DEDBT), by Rhodococcus sp. stra...

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Bibliographic Details
Published in:Environmental science & technology 1998-02, Vol.32 (3), p.421-426
Main Authors: MacPherson, Tanya, Greer, Charles W, Zhou, Edward, Jones, Alison M, Wisse, Gesine, Lau, Peter C. K, Sankey, Bruce, Grossman, Matthew J, Hawari, Jalal
Format: Article
Language:English
Subjects:
Applied sciences
Chemistry
Constitution and properties of crude oils, shale oils, natural gas and bitumens. Analysis and characteristics
Crude oil, natural gas and petroleum products
Energy
Exact sciences and technology
Extraction
Fuels
Gas chromatography
Hydrocarbons
Mass spectrometry
Metabolites
Microorganisms
Organic chemistry
Rhodococcus
Sulfur
Sulfur compounds
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Summary:A combined solid-phase microextraction/GC-MS analytical technique was used to monitor the formation of metabolites in the biodesulfurization of the bitumen model organosulfur compounds, dibenzothiophene (DBT) and the dialkylated derivative 4,6-diethyldibenzothiophene (DEDBT), by Rhodococcus sp. strain ECRD-1. In the case of DBT, the following metabolites were detected:  DBT 5-oxide (sulfoxide), DBT 5,5-dioxide (sulfone), dibenz[c,e][1,2]oxathiin 6-oxide (sultine), dibenz[c,e][1,2]oxathiin 6,6-dioxide (sultone), and the end product, 2-hydroxybiphenyl (2-HBP), whereas, with DEDBT, 4,6-DEDBT 5-oxide, 4,6-diethyldibenz[c,e][1,2]oxathiin 6-oxide (sultine), and 2-hydroxy-3,3‘-diethylbiphenyl (HDEBP) as the final product, were identified. A time course study for the formation and disappearance of DBT and DEDBT metabolites was used to construct desulfurization pathways, which in both cases, involved the formation of the corresponding sulfoxides.
ISSN:0013-936X
1520-5851
DOI:10.1021/es970356j