A new selective optode for the determination of iron(III) based on the immobilization of morin on triacetylcellulose: A combined experimental and computational study

• Published in: Materials Science & Engineering C 2019-01, Vol.94, p.410-416
• Main Authors: Firooz, A.R., Movahedi, M., Sabzyan, H.
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Anisotropy; Binding; Biosensing Techniques; Calibration; Carbides; Carbon; Cellulose - analogs & derivatives; Cellulose - chemistry; Computer applications; Computer Simulation; Density functional theory; DFT; Drinking water; Fe(III) ion; Flavonoids - chemistry; Humans; Immobilization; Iron; Iron - blood; Iron(III) optical sensor; Materials science; Metal ions; Molecular Conformation; Morin; Optical measuring instruments; Optode; Organic chemistry; Pharmaceuticals; Selectivity; Serum iron; Spectrophotometry; Spectrophotometry, Ultraviolet; Tablets; Triacetylcellulose; Water - chemistry; Water analysis; Water sampling
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2018.09.031

Accurate and fast measurement of the iron ion in biological, pharmaceutical and medical samples is of great applied importance. In this work, a novel optical sensor (optode) for the Fe(III) ion is fabricated based on the immobilization of morin (2′,3,4′,5,7-pentahydroxyflavone) on a triacetylcellulose membrane. Chemical binding of the Fe(III) ion with the immobilized morin is monitored spectrophotometrically at 334 nm. The prepared optode shows excellent response over a wide range of concentrations from 1.06 × 10−10 to 4.73 × 10−5 M with a detection limit of 4.23 × 10−11 M Fe(III). Effects of the factors determining sensitivity of the optode are studied and optimized. The prepared optical sensor shows good selectivity toward the Fe(III) ion in the presence of a number of other metal ions. The developed sensor is applied successfully and satisfactorily for the determination of iron in three pharmaceutical, one plasma and two serum samples. In addition, concentration of the Fe(III) ion in two tap water samples is measured using standard addition method. Density functional theory (TD) B3LYP/6-311++G** method is used to investigate structure and binding characteristics, and calculate the UV–Vis spectrum of the Fe(III)-morin complex. •A novel optode for Fe(III) is fabricated based on immobilized morin (2',3,4',5,7-pentahydroxyflavone) on triacetylcellulose membrane.•This optode shows excellent selectivity/response to Fe(III) for 1.06×10-10 – 4.73×10-5 M concentrations (detection limit: 4.23×10-11 M).•The developed optode is applied successfully for determination of iron in pharmaceutical, plasma, serum and tap water samples.•(TD) B3LYP/6-311++G** method is used to optimize structure and calculate properties (UV-Vis spectrum) of the Fe(III)-morin complex.