Cytotoxic and cell cycle arrest induction of pentacyclic triterpenoides separated from Lantana camara leaves against MCF-7 cell line in vitro

• Published in: Molecular biology reports 2019-02, Vol.46 (1), p.381-390
• Main Authors: Shamsee, Zahraa R., Al-Saffar, Ali Z., Al-Shanon, Ahmed F., Al-Obaidi, Jameel R.
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Antioxidants; Antioxidants - pharmacology; Ascorbic acid; Biomedical and Life Sciences; Cancer; Caspase; Caspase-9; Cell cycle; Cell Cycle Checkpoints - drug effects; Chromatography, Liquid; Cytotoxicity; Histology; Humans; Lantana - metabolism; Lantana - physiology; Lantana camara; Leaves; Life Sciences; Lymphocytes B; MCF-7 Cells - drug effects; Medicinal plants; Morphology; Oleanolic Acid - analogs & derivatives; Oleanolic Acid - isolation & purification; Original Article; Pentacyclic Triterpenes - isolation & purification; Pentacyclic Triterpenes - pharmacology; Pentacyclic triterpenoids; Plant extracts; Plant Extracts - chemistry; Plant Leaves - chemistry; S phase; Tandem Mass Spectrometry; Triterpenes - pharmacology; Triterpenoids; Tumors
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-018-4482-3

Lantana camara is an important medicinal plant that contains many active compounds, including pentacyclic triterpenoids, with numerous biological activities. The present study was conducted to evaluate the anti-oxidant, anti-tumour, and cell cycle arrest properties of chemical compounds extracted from L. camara leaves. Four compounds were identified after subjecting the plant methanolic extract to LC-MS/MS analysis: lantadene A, lantadene B, icterogenin, and lantadene C. Potential antioxidant activity was examined using 2, 2-diphenyl-1-picrylhydrazyl and compared with vitamin C as a control. Lantadene A and B were confirmed to possess the highest scavenging activity, while icterogenin and lantadene C exhibited a lesser antioxidant effect. All extracted compounds exerted a dose-dependent reduction in MCF-7 cell viability; however, lantadene B showed the highest anti-cancer activity, with an IC 50 of 112.2 μg mL −1 , and was therefore used in subsequent experiments. The results also confirmed the significant release of caspase 9 in a dose-dependent pattern following treatment of MCF-7 cells with a range of lantadene B concentrations. Lantadene B was found to induce MCF-7 cell cycle arrest in G1, blocking the G1/S transition with a maximum significant ( p  ≤ 0.01) cell count of 80.35% at 25 µg mL −1 . No significant changes were observed in S phase, but a decrease in the MCF-7 population was exhibited in G2/M phase.