Chemometric analysis of porcine, bovine and ovine heparins

•1H-NMR of heparins from different animals show distinct clusters in PCA and k means analysis.•Disaccharides components of heparins from different animals show distinct clusters in PCA and k means.•Tetrasaccharides components of heparins from different animals show distinct clusters in PCA and k mea...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2019-02, Vol.164, p.345-352
Main Authors: Ouyang, Yilan, Han, Xiaorui, Yu, Yanlei, Chen, Jianle, Fu, Li, Zhang, Fuming, Linhardt, Robert J., Fareed, Jawed, Hoppensteadt, Debra, Jeske, Walter, Kouta, Ahmed, Zhang, Zhenqing, Xia, Ke
Format: Article
Language:English
Subjects:
1H NMR spectroscopy
Animal origin
Chemometrics
Classification
Heparin
Mass spectrometry
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Summary:•1H-NMR of heparins from different animals show distinct clusters in PCA and k means analysis.•Disaccharides components of heparins from different animals show distinct clusters in PCA and k means.•Tetrasaccharides components of heparins from different animals show distinct clusters in PCA and k means.•Chemometric analysis could help predict the heparin animal source and detect blending. Heparin is a polysaccharide anticoagulant drug isolated from animal tissues. There have been concerns on the safety and security of the heparin supply chain since 2007-8 when a contamination crisis led to its disruption. The current study applies a suite of modern analytical techniques to porcine, bovine and ovine intestinal mucosal heparins. These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin’s antithrombin III binding site. Chemometric analysis was then applied to understand how these structural differences to predict the animal/tissue source of heparin and to help detect blending of heparins from various sources.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2018.10.052