Overexpression of CAV3 facilitates bone formation via the Wnt signaling pathway in osteoporotic rats

• Published in: Endocrine 2019-03, Vol.63 (3), p.639-650
• Main Authors: Yang, Run-Bao, Lin, Feng-Fei, Yang, Jun, Chen, Bin, Zhang, Ming-Hua, Lu, Qiao-Ping, Xiao, Bo, Liu, Yan, Zheng, Ke, Qiu, Yong-Rong
• Format: Article
• Language: English
• Subjects: Alkaline Phosphatase - blood; Animals; Bone and Bones - metabolism; Bone and Bones - pathology; Caveolin 3 - metabolism; Female; Gene Silencing; Osteogenesis; Osteoporosis - metabolism; Osteoporosis - pathology; Osteoporosis - therapy; Rats, Wistar; RNA, Small Interfering - therapeutic use; Tartrate-Resistant Acid Phosphatase - blood; Wnt Signaling Pathway
• ISSN: 1559-0100
• DOI: 10.1007/s12020-018-1803-1

Osteoporosis is a condition characterized by decreased bone density and bone strength, commonly observed among older individuals. Caveolin-3 (CAV3) is a principal structural protein of the caveolae membrane domains, which has been reported to participate in cell signaling as well as the maintenance of cell structure. The aim of the current study was to investigate the effects involved with the silencing of CAV3 on bone formation among osteoporotic rat models via the Wnt signaling pathway. Osteoporosis was initially induced by means of ovariotomy among rat models in order to determine the expression of CAV3. Then, to confirm the specific function and mechanism of CAV3 from an osteoporosis perspective, the CAV3 expression vector was constructed and transfected into the osteoblasts of the osteoporotic rats. Afterward, the mRNA and protein expressions of CAV3, β-catenin, low-density lipoprotein receptor-related protein 5 (LRP5), T-cell factor (TCF), and Wnt3a in addition to cell proliferation and apoptosis were detected accordingly. Positive expression of CAV3 exhibited diminished levels in the bone tissues of osteoporotic rats. The osteoblasts of the osteoporotic rats treated with overexpressed CAV3 displayed elevated mRNA and protein expression levels of β-catenin, LRP5, TCF, and Wnt3a. Increased cell proliferation and decreased cell apoptosis were also observed, while the osteoblasts of the osteoporotic rats treated with si-CAV3 exhibited an opposite result. Overexpressed CAV3 promotes bone formation and suppresses the osteoporosis progression via the activation of the Wnt signaling in rat models, suggesting CAV3 as a potential target biomarker in the treatment of osteoporosis.