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FLI1 Exonic Circular RNAs as a Novel Oncogenic Driver to Promote Tumor Metastasis in Small Cell Lung Cancer
The aberrantly upregulated Friend leukemia virus integration 1 ( ) is closely correlated with the malignant phenotype of small cell lung cancer (SCLC). It is interesting to note that the CRISPR gene knockout by Cas9 gRNAs that target the coding region and the posttranscriptional knockdown by shRNAs...
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Published in: | Clinical cancer research 2019-02, Vol.25 (4), p.1302-1317 |
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Main Authors: | , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The aberrantly upregulated Friend leukemia virus integration 1 (
) is closely correlated with the malignant phenotype of small cell lung cancer (SCLC). It is interesting to note that the CRISPR gene knockout by Cas9 gRNAs that target the
coding region and the posttranscriptional knockdown by shRNAs that target the 3' region of
mRNA yielded distinct antimetastasis effects in SCLC cells. This study attempts to examine if
exonic circular RNAs (FECR) function as a new malignant driver that determines the metastatic phenotype in SCLC.
The clinical relevance of FECRs was examined in 56 primary SCLC tissues and 50 non-small cell lung cancer (NSCLC) tissues. The prognostic value of FECRs was examined by measuring serum exosomal FECRs in a longitudinal cohort of patients with SCLC. The oncogenic activity of FECRs was investigated in both SCLC cell lines and animal xenograft studies. Finally, we explored the molecular mechanisms underlying these noncoding RNAs as a malignant driver.
Therapeutic comparison of CRISPR Cas9 knockout and shRNA knockdown of
identified FECRs as a new noncanonical malignant driver in SCLC. Using RNA FISH and quantitative PCR, we found that FECR1 (exons 4-2-3) and FECR2 (exons 5-2-3-4) were aberrantly upregulated in SCLC tissues (
< 0.0001), and was positively associated with lymph node metastasis (
< 0.01). Notably, serum exosomal FECR1 was associated with poor survival (
= 0.038) and clinical response to chemotherapy. Silencing of FECRs significantly inhibited the migration in two highly aggressive SCLC cell lines and reduced tumor metastasis
. Mechanistically, we uncovered that FECRs sequestered and subsequently inactivated tumor suppressor miR584-3p, leading to the activation of the Rho Associated Coiled-Coil Containing Protein Kinase 1 gene (ROCK1).
This study identifies
exonic circular RNAs as a new oncogenic driver that promotes tumor metastasis through the miR584-ROCK1 pathway. Importantly, serum exosomal FECR1 may serve as a promising biomarker to track disease progression of SCLC. |
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ISSN: | 1078-0432 1557-3265 |
DOI: | 10.1158/1078-0432.ccr-18-1447 |