Chemical modification of polystyrene's surface and its effect on immobilized antibodies

The quantity and immunoreactivity of antibodies coupled to treated polystyrene beads were studied as a function of the chemical treatments used to prepare the polystyrene surface. Injection-molded polystyrene beads were nitrated with sulfuric and nitric acids followed by reduction to form amine grou...

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Bibliographic Details
Published in:Colloids and surfaces. A, Physicochemical and engineering aspects Physicochemical and engineering aspects, 1998-01, Vol.132 (2-3), p.193-201
Main Authors: Page, J.D., Derango, R., Huang, A.E.
Format: Article
Language:English
Subjects:
Amination
Antibodies
Antibody activity
Antibody immobilization
Antigen-antibody reactions
Chemical modification
Composition effects
Grafting (chemical)
Injection molding
Nitration
Nitration and amination
Polystyrene
Surface analysis
Surface structure
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Summary:The quantity and immunoreactivity of antibodies coupled to treated polystyrene beads were studied as a function of the chemical treatments used to prepare the polystyrene surface. Injection-molded polystyrene beads were nitrated with sulfuric and nitric acids followed by reduction to form amine groups. The nitration conditions used affect the quantity of nitro and amine groups introduced, and ultimately the quantity of antibody coupled to the surface. Aminated beads were also grafted with monomethoxypolyethylene glycol (mPEG) prior to antibody coupling to determine if this modification could lower non-specific protein binding to the beads. At high surface levels of mPEG, antibody coupling was completely blocked. At lower surface levels of mPEG, antibody coupling was accomplished, but with no reduction of the non-specific protein binding to the beads.
ISSN:0927-7757
1873-4359
DOI:10.1016/S0927-7757(97)00176-3