A lactoperoxidase-based flow injection amperometric biosensor for iodide detection

The following contains work carried out into the preparation of an amperometric enzymatic biosensor (M.P. Byfield, R.A. Abuknesha, Biochemical aspects of biosensors. Biosens. Bioelectron. 9 (1994) 373–400; C.R. Lowe, An introduction to the concepts and technology of biosensors. Biosensors 1 (1985) 3...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 1998-04, Vol.47 (1), p.30-36
Main Authors: Desmond, Donal, Compagnone, Dario, Lane, Bill, Alderman, John, Glennon, J.D, Guilbault, G.G
Format: Article
Language:English
Subjects:
Amperometric enzymatic biosensor
Amperometric sensors
Biosensors
Electrochemical transduction
Electrochemistry
Iodine compounds
Lactoperoxidase
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Summary:The following contains work carried out into the preparation of an amperometric enzymatic biosensor (M.P. Byfield, R.A. Abuknesha, Biochemical aspects of biosensors. Biosens. Bioelectron. 9 (1994) 373–400; C.R. Lowe, An introduction to the concepts and technology of biosensors. Biosensors 1 (1985) 3–16; J.S. Schultz, Biosensors. Sci. Am. 8 1991 48–55) using the enzyme, lactoperoxidase (LPOD), for the detection of iodide. In this paper, a study of two major avenues which were undertaken to achieve an iodide biosensor, using electrochemical transduction techniques, are discussed. The first of these focuses on the use of LPOD in solution using both DPBS and acetate buffers, while later work has concentrated on investigations into the immobilised enzyme. The former has been shown to measure iodide concentrations of less than 0.1 μmol/l while the latter can be used to detect iodide levels lower than 10 μmol/l. The difficulties and drawbacks associated with both these systems are discussed.
ISSN:0925-4005
1873-3077
DOI:10.1016/S0925-4005(98)00049-5