Electrostatics of DNA-cationic lipid complexes : isoelectric instability

We propose a Poisson-Boltzmann electrostatic theory for DNA/cationic lipid complexes modeled as a stack of aligned DNA chains intercalated with lipid bilayers, a structure suggested by the recent X-ray synchrotron studies of Radler et al. Poisson-Boltzmann theory is shown to predict that the isoelec...

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Bibliographic Details
Published in:The European physical journal. B, Condensed matter physics Condensed matter physics, 1998-07, Vol.4 (1), p.75-88
Main Author: BRUINSMA, R
Format: Article
Language:English
Subjects:
Absorption
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Cell membranes
Cytology
Dna, deoxyribonucleoproteins
Electric charge
Electrostatics
Entropy
Fundamental and applied biological sciences. Psychology
Intercalation compounds
Lipids
Nucleic acids
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Summary:We propose a Poisson-Boltzmann electrostatic theory for DNA/cationic lipid complexes modeled as a stack of aligned DNA chains intercalated with lipid bilayers, a structure suggested by the recent X-ray synchrotron studies of Radler et al. Poisson-Boltzmann theory is shown to predict that the isoelectric point - where the DNA and cationic lipid charges are in balance - is unstable against absorption of extra DNA or lipid material. The instability is caused by the entropy gain obtained following the release of small ions inside the complex and is manifested by singular behavior of the rod-rod spacing near the isoelectric point. We apply the theory to a discussion of the results of Radler et al.
ISSN:1434-6028
1434-6036
DOI:10.1007/s100510050353