Response of antibiotic resistance genes abundance by graphene oxide during the anaerobic digestion of swine manure with copper pollution

The pollution of various environments with antibiotic resistance genes (ARGs) is an urgent problem that needs to be addressed, especially in heavy metal-polluted environments. This study investigated the responses of ARGs and mobile genetic elements (MGEs) to the addition of graphene oxide (GO) to s...

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Bibliographic Details
Published in:The Science of the total environment 2019-03, Vol.654, p.292-299
Main Authors: Zhang, Ranran, Gu, Jie, Wang, Xiaojuan, Li, Yang, Liu, Jiayao, Lu, Chunya, Qiu, Ling
Format: Article
Language:English
Subjects:
Anaerobic digestion
Anaerobiosis
Animals
Antibiotic resistance gene
Copper - metabolism
Copper - toxicity
Drug Resistance, Microbial - drug effects
Drug Resistance, Microbial - genetics
Environmental Pollution - prevention & control
Genes, Bacterial - drug effects
Graphene oxide
Graphite - chemistry
Manure - analysis
Manure - microbiology
Metal resistance gene
Microbiota - drug effects
Microbiota - genetics
Mobile genetic element
Swine
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Summary:The pollution of various environments with antibiotic resistance genes (ARGs) is an urgent problem that needs to be addressed, especially in heavy metal-polluted environments. This study investigated the responses of ARGs and mobile genetic elements (MGEs) to the addition of graphene oxide (GO) to swine manure containing a high concentration copper during anaerobic digestion. The total copy numbers of ARGs and MGEs were significantly enhanced by the pressure due to Cu. GO significantly decreased the ARG and MGE copy numbers, where the low GO concentration performed better than the high GO concentration. Network analysis showed that most of the ARGs and MGEs co-occurred and they shared the same major potential host bacteria. The contributions of different factors to ARG abundances were assessed by redundancy analysis and MGEs had the most important effect on the fate of ARGs. Thus, GO may reduce the abundance of ARGs mainly by removing MGEs. [Display omitted] •GO was effective in reducing the copy numbers of ARGs and MGEs.•The abundance of ARGs was reduced mainly by GO hindering the horizontal transfer.•The contribution of environmental to ARGs was through bacterial community.•Potential host bacteria for MRGs have significant correlations with most ARGs.
ISSN:0048-9697
1879-1026
DOI:10.1016/j.scitotenv.2018.11.094