Diversity of endophytic fungi of Paeonia lactiflora Pallas and screening for fungal paeoniflorin producers

Endophytic fungi from Paeonia lactiflora Pallas, which is mainly distributed in China, were characterized and screened to identify those capable of producing paeoniflorin. A total of 101 isolates obtained from the roots, stems and leaves of P. lactiflora were grouped into 16 fungal taxa based on mor...

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Bibliographic Details
Published in:FEMS microbiology letters 2018-12, Vol.365 (24), p.1
Main Authors: Cheng, Xiaozhong, Wei, Zhiwen, Pu, Shunchang, Xiang, Min, Yan, Aolei, Zhang, Yu, Wang, Xiaomei
Format: Article
Language:English
Subjects:
Ascomycota
Biodiversity
Biosynthesis
Botanical research
China
Endophytes
Endophytes - classification
Endophytes - genetics
Endophytes - isolation & purification
Endophytes - metabolism
Fermentation
Fungi
Fungi - classification
Fungi - genetics
Fungi - isolation & purification
Fungi - metabolism
Glucosides - biosynthesis
High performance liquid chromatography
Identification and classification
Industrial production
Leaves
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Microbiology
Monoterpenes
Paeonia - microbiology
Paeonia lactiflora
Peonies
Phylogeny
Physiological aspects
Phytochemicals
Plant Leaves - microbiology
Plant Roots - microbiology
Plant Stems - microbiology
Roots
Stems
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Summary:Endophytic fungi from Paeonia lactiflora Pallas, which is mainly distributed in China, were characterized and screened to identify those capable of producing paeoniflorin. A total of 101 isolates obtained from the roots, stems and leaves of P. lactiflora were grouped into 16 fungal taxa based on morphological traits and internal transcribed spacers sequences, indicating that endophytic fungi of P. lactiflora are abundant and diverse. The dominant endophytic fungi were Aspergillus, Alternaria and Penicillium. More fungi were recovered from leaves than from roots and stems. The similarity index was highest between the stems and leaves (0.733), followed by the roots and leaves (0.615) and the stems and roots (0.563). Analyses of the fermentation extracts of 22 endophytic fungi by high-performance liquid chromatography and mass spectrometry revealed that three strains (R12, Alternaria tenuissima; S4, Aspergillus flavus; and R17 Penicillium commune) were able to produce paeoniflorin. Among the paeoniflorin-producing fungi, the yield of paeoniflorin from A. flavus S4 was 342.4 μg/L, and this strain could be used as a candidate for the industrial production of paeoniflorin.
ISSN:1574-6968
0378-1097
1574-6968
DOI:10.1093/femsle/fny263