Three-phase partitioning for the direct extraction and separation of bioactive exopolysaccharides from the cultured broth of Phellinus baumii

• Published in: International journal of biological macromolecules 2019-02, Vol.123, p.201-209
• Main Authors: Wang, Yao-Yao, Ma, Haile, Ding, Zhi-Chao, Yang, Yan, Wang, Wen-Han, Zhang, He-Nan, Yan, Jing-Kun
• Format: Article
• Language: English
• Subjects: Exopolysaccharide; Phellinus baumii; Three-phase partitioning
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2018.11.065

In this study, three-phase partitioning (TPP) was used to directly extract and separate bioactive exopolysaccharides (EPSs) from a cultured broth of Phellinus baumii. The maximum extraction yield of EPS was 52.09% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.0:1.5 (v/v) ratio of cultured broth to t‑butanol, 30 min, and 35 °C. A multifrequency power ultrasound in a sequential mode coupled with TPP resulted in ~9.12% increment in extraction yield and ~80% reduction in extraction time compared with those of traditional TPP. The carbohydrate (88.21%) and uronic acid (3.37%) contents of partially purified EPS were higher than those of EPS-C obtained through conventional ethanol precipitation and separation methods. EPS and EPS-C exhibited similar preliminary structural characteristics and different monosaccharide compositions and molecular weights. The radical-scavenging abilities, antioxidant capacities, α‑amylase and α‑glycosidase inhibitory activities, and macrophage stimulation activities of EPS were also higher than those of EPS-C. Therefore, it could be concluded that TPP as a simple and green separation technique could be used to directly extract and separate bioactive EPS from the fermentation broths of mushrooms and other fungi. •EPS from a cultured broth of P. baumii was directly extracted and separated by TPP.•The highest extraction yield of EPS was 52.09% under the optimized conditions.•EPS exhibited preferable physicochemical properties in comparison with EPS-C.•EPS obtained from TPP showed stronger biological activities than that of EPS-C.