Integrated reversed micellar extraction and stripping of α-amylase

• Published in: Biotechnology and bioengineering 1999-06, Vol.63 (5), p.583-592
• Main Authors: Lazarova, Z., Tonova, K.
• Format: Article
• Language: English
• Subjects: Ammonium compounds; Biological and medical sciences; bioseparation; Biotechnology; Catalyst activity; downstream processes; Enzyme engineering; Enzyme kinetics; enzyme purification; Fundamental and applied biological sciences. Psychology; Improved methods for extraction and purification of enzymes; Mass transfer; Methods. Procedures. Technologies; Micelles; Organic solvents; protein extraction; Purification; reversed micelles; Solvent extraction; Surface active agents; α-amylase
• ISSN: 0006-3592; 1097-0290
• DOI: 10.1002/(SICI)1097-0290(19990605)63:5<583::AID-BIT8>3.0.CO;2-U

In this work the possibility of integrating the two process steps—extraction of an enzyme from its source aqueous solution into an organic solvent containing reversed micelles and its stripping into another aqueous medium of appropriate composition is studied. The kinetics of enzyme mass transfer during the integrated process is investigated using a stirred cell with two separated compartments. The enzyme/surfactant system chosen consisted of α‐amylase and the cationic surfactant CTAB (cetyl trimethyl ammonium bromide). Comparison between the classical extraction process and the “integrated reversed micellar extraction–stripping” is made. The enzyme activity changes during the transfer process are followed, and the registered tendencies are discussed. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 63: 583–592, 1999.