Effects of UVB and UVC irradiation on cariogenic bacteria in vitro

The aim of this study was to evaluate the bactericidal effect of ultraviolet (UV) light at 265-nm (UVC) and 310-nm (UVB) wavelengths from a newly developed UV light-emitting diode (LED) device against cariogenic bacteria in vitro. Suspensions of Streptococcus mutans ( S. mutans ) and Streptococcus s...

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Bibliographic Details
Published in:Lasers in medical science 2019-07, Vol.34 (5), p.981-989
Main Authors: Uchinuma, Shigeki, Shimada, Yasushi, Matin, Khairul, Hosaka, Keiichi, Yoshiyama, Masahiro, Sumi, Yasunori, Tagami, Junji
Format: Article
Language:English
Subjects:
Bacteria
Colonies
Demineralization
Demineralizing
Dental caries
Dentin
Dentistry
Fluorescence
Fluorescence microscopy
Irradiation
Lasers
Light emitting diodes
Medicine
Medicine & Public Health
Optical density
Optical Devices
Optics
Original Article
Photonics
Quantum Optics
Streptococcus infections
Ultraviolet radiation
Wavelengths
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Summary:The aim of this study was to evaluate the bactericidal effect of ultraviolet (UV) light at 265-nm (UVC) and 310-nm (UVB) wavelengths from a newly developed UV light-emitting diode (LED) device against cariogenic bacteria in vitro. Suspensions of Streptococcus mutans ( S. mutans ) and Streptococcus sobrinus were directly irradiated by UVB or UVC for 2.5 min or 5 min. Numbers of colonies were counted and calculated as colony forming units (CFU) per milliliter. Fluorescence microscopy (FM) and optical density measurements at 490 nm (OD 490 ) were also taken after irradiation. In addition, the bactericidal effects of irradiation against S. mutans under 0.5 mm-thick dentin were compared using culture tests and OD 490 measurements. Direct UV-LED irradiation with both UVB and UVC showed strong bactericidal effects. UVB showed superior bactericidal effect through 0.5-mm-thick dentin than did UVC, especially after demineralization. These results suggest that UVB irradiation could be utilized for the prevention and management of dental caries.
ISSN:0268-8921
1435-604X
DOI:10.1007/s10103-018-2685-4