Characterization and function of a group II type I interferon in the perciform fish, large yellow croaker (Larimichthys crocea)

• Published in: Fish & shellfish immunology 2019-03, Vol.86, p.152-159
• Main Authors: Ding, Yang, Guan, Yanyun, Huang, Xiaohong, Ao, Jingqun, Chen, Xinhua
• Format: Article
• Language: English
• Subjects: Antiviral activity; Expression modulation; Interferon c; Large yellow croaker (Larimichthys crocea); Type I IFN response
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2018.11.036

Teleost fish possess two groups of type I interferons (IFNs) with two (group I IFNs) or four (group II IFNs) conserved cysteines, which are further classified into seven subgroups. In our previous study, two group I type I IFNs, LcIFNd and LcIFNh (a new subgroup member), were identified in the perciform fish, large yellow croaker (Larimichthys crocea). Here, we identified a group II type I IFN, LcIFNc, in this species. The deduced LcIFNc contained six cysteines, four of which are highly conserved (C1: C28, C2:C53, C3: C130, and C4:C159) in the fish group II type I IFNs, and a typical type I IFN signature motif was also found in it. Phylogenetic analysis indicated that LcIFNc belongs to the IFNc subgroup of fish group II type I IFNs. LcIFNc was constitutively expressed in all examined tissues, and was rapidly up-regulated in spleen and head kidney by poly(I:C) and Aeromonas hydrophila. Recombinant LcIFNc protein (rLcIFNc) could increase the expression of antiviral genes, Mx1, PKR and ISG15, in large yellow croaker peripheral blood leukocytes (PBLs). The rLcIFNc also exhibited obvious antiviral activity based on less cytopathic effect (CPE) and decreased expression levels of several viral genes in the rLcIFNc-treated grouper spleen (GS) cells following Singapore grouper iridovirus (SGIV) infection. Additionally, rLcIFNc was able to induce the expression of LcIFNc, as well as LcIFNd and LcIFNh in the PBLs and primary head kidney cells (HKCs) from large yellow croaker. These results therefore indicated that LcIFNc not only had antiviral activity, but also mediated the regulation of type I IFN response. •We identified a group II type I IFN, LcIFNc, in large yellow croaker Larimichthys crocea.•LcIFNc was constitutively expressed in all examined tissues, and rapidly up-regulated by poly(I:C) and Aeromonas hydrophila.•LcIFNc exhibited obvious antiviral activity.•LcIFNc mediated the regulation of type I IFN response by inducing the expression of itself and LcIFNd and LcIFNh.