ACTL6A interacts with p53 in acute promyelocytic leukemia cell lines to affect differentiation via the Sox2/Notch1 signaling pathway

• Published in: Cellular signalling 2019-01, Vol.53, p.390-399
• Main Authors: Zhong, Peng-Qiang, Zhong, Liang, Yao, Juan-Juan, Liu, Dong-Dong, Yuan, Zhen, Liu, Jun-Mei, Chen, Min, Yao, Shi-Fei, Zhao, Yi, Liu, Lu, Li, Lian-Wen, Liu, Bei-Zhong
• Format: Article
• Language: English
• Subjects: Actin-like 6A; Actins - metabolism; Acute promyelocytic leukemia; Cell Line, Tumor; Chromosomal Proteins, Non-Histone - metabolism; DNA-Binding Proteins - metabolism; HL-60 Cells; Humans; Leukemia, Promyelocytic, Acute - metabolism; Notch1; Protein Interaction Maps; Receptor, Notch1 - metabolism; Signal Transduction; Sox2; SOXB1 Transcription Factors - metabolism; Tumor Suppressor Protein p53 - metabolism
• ISSN: 0898-6568; 1873-3913
• DOI: 10.1016/j.cellsig.2018.11.009

Actin-like 6A (ACTL6A), a component of BAF chromatin remodeling complexes, is important for cell differentiation. Nevertheless, its role and mechanism in acute promyelocytic leukemia (APL) has not been reported. To identify the genes that may participate in the development of APL, we analyzed data from an APL cDNA microarray (GSE12662) in the NCBI database, and found that ACTL6A was up-regulated in APL patients. Subsequently, we investigated the function and mechanisms of ACTL6A in myeloid cell development. The expression of ACTL6A was gradually decreased during granulocytic differentiation in all-trans retinoic acid-treated NB4 and HL-60 cells, and phorbol myristate acetate-treated HL-60 cells. We also found that knockdown of ACTL6A promoted differentiation in NB4 and HL-60 cells, and decreased the levels of Sox2 and Notch1. Mechanistically, ACTL6A interacted with and was co-localized with Sox2 and p53. Meanwhile, CBL0137, an activator of p53, decreased the expression of ACTL6A and promoted differentiation in NB4 and HL-60 cells. These findings suggest that the inhibition of ACTL6A promotes differentiation via the Sox2 and Notch1 signaling pathways. Furthermore, the differentiation promoted by inhibiting ACTL6A could be regulated by p53 via its physical interaction with ACTL6A. •The relation between ACTL6A and differentiation of APL cells is reported for the first time.•Knockdown of ACTL6A promotes differentiation via Sox2 and the Notch1 signaling pathway.•CBL0137, a p53 activator, promotes differentiation via a physical interaction between p53 and ACTL6A.•Inhibitors of ACTL6A and Notch1 signaling are potential therapeutics for APL.