Comparative Nuclear Proteomics Analysis Provides Insight into the Mechanism of Signaling and Immune Response to Blast Disease Caused by Magnaporthe oryzae in Rice

Modulation of plant immune system by extrinsic/intrinsic factors and host‐specific determinants fine‐tunes cellular components involving multiple organelles, particularly nucleus to mount resistance against pathogen attack. Rice blast, caused by hemibiotrophic fungus Magnaporthe oryzae, is one of th...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2019-02, Vol.19 (3), p.e1800188-n/a
Main Authors: Narula, Kanika, Choudhary, Pooja, Ghosh, Sudip, Elagamey, Eman, Chakraborty, Niranjan, Chakraborty, Subhra
Format: Article
Language:English
Subjects:
2‐DE coupled mass spectrometry
Cell division
Chromatin
comparative proteomics
Correlation analysis
Deprivation
Disease Resistance
Fungi
Gene expression
Gene regulation
Genotypes
Glycolysis
Homeostasis
Host-Pathogen Interactions
Immune response
Immune system
Immunity
Immunomodulation
Magnaporthe - immunology
Magnaporthe - physiology
Magnaporthe oryzae
Network analysis
Nuclei (cytology)
nucleus
Organelles
Oryza
Oryza - immunology
Oryza - microbiology
Plant Diseases - immunology
Plant Diseases - microbiology
Plant Immunity
Plant Proteins - immunology
protein networks
Proteins
Proteomics
Rice
Rice blast
Signal Transduction
Signaling
Tandem Mass Spectrometry
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Summary:Modulation of plant immune system by extrinsic/intrinsic factors and host‐specific determinants fine‐tunes cellular components involving multiple organelles, particularly nucleus to mount resistance against pathogen attack. Rice blast, caused by hemibiotrophic fungus Magnaporthe oryzae, is one of the most devastating diseases that adversely affect rice productivity. However, the role of nuclear proteins and their regulation in response to M. oryzae remains unknown. Here, the nucleus‐associated immune pathways in blast‐resistant rice genotype are elucidated. Temporal analysis of nuclear proteome is carried out using 2‐DE coupled MS/MS analysis. A total of 140 immune responsive proteins are identified associated with nuclear reorganization, cell division, energy production/deprivation, signaling, and gene regulation. The proteome data are interrogated using correlation network analysis that identified significant functional modules pointing toward immune‐related coinciding processes through a common mechanism of remodeling and homeostasis. Novel clues regarding blast resistance include nucleus‐associated redox homeostasis and glycolytic enzyme–mediated chromatin organization which manipulates cell division and immunity. Taken together, the study herein provides evidence that the coordination of nuclear function and reprogramming of host translational machinery regulate resistance mechanism against blast disease.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.201800188